正交实验法优化碧螺春茶叶中总黄酮提取工艺.pdfVIP

第46卷第1期 应　 用　 化　 工 Vol.46 No.1 2017年1月 Applied Chemical Industry Jan.2017 正交实验法优化碧螺春茶叶中总黄酮提取工艺 1,2 3 1 1,2 1,2 刘映 ,王敏 ,谢秀珍 ,彭俊梅 ,唐国涛 (1.南华大学 药学与生物科学学院药学系,湖南 衡阳　 421001;2.湖南省分子靶标新药研究协同创新中心, 湖南 衡阳　 421001;3.南华大学 船山学院,湖南 衡阳　 421001) 摘　 要:用乙醇提取碧螺春茶叶中的总黄酮,考察乙醇浓度、提取温度、提取时间、料液比对总黄酮提取率的影响。 结果表明,各因素影响主次顺序为乙醇浓度提取温度料液比提取时间;最佳提取工艺为:乙醇浓度60%,提 取温度60 ℃,料液比1∶40 g/ mL,提取时间2.5 h。 在此条件下,碧螺春茶叶中总黄酮提取率为15.7%。 关键词:碧螺春茶叶;总黄酮;乙醇浸提;提取率 中图分类号:TQ914.1;TQ460.4;TQ610.4　 　 文献标识码:A　 　 文章编号:1671-3206(2017)01-0074-03 Optimization the extraction of total flavonoids from Biluochun tea by orthogonal test 1,2 3 1 1,2 1,2 LIU Ying ,WANGMin ,XIEXiu-zhen ,PENGJun-mei ,TANG Guo-tao (1.Department of Pharmacy,School of Pharmaceutical and Biological Science,University of South China,Hengyang 421001,China;2.Hunan Province Cooperative Center for Molecular Target New Drug Study,Hengyang421001,China; 3.Chuanshan College,University of South China,Hengyang421001,China) Abstract:Using alcohol to extract total flavonoid from Biluochun tea.The influence of ethanol concentra- tion,extraction time,extraction temperature,liquid to material ratio on total flavonoids was investigated. Results showedthattheinfluenceof variousfactorsorder isethanol concentrationextractiontemperature liquid to material ratio extraction time,and the best process conditions is when extract ethanol is 60%,ultrasonic time is2.5 h,liquidto material ratio is 1∶40 g/ mL,extractiontemperature is60 ℃.Un- der this condition,the extraction rate was 15.7%. Key words:Bilu