热脱附气质联用法对荷叶挥发油成分的对比分析ComparativeAnalysis.PDF

2009 年第67 卷 化 学 学 报 Vol. 67, 2009 第20 期, 2368～2374 ACTA CHIMICA SINICA No. 20, 2368～2374 ·研究论文· 吹扫/捕集-热脱附气质联用法对荷叶挥发油成分的对比分析 赟 *,a a,b a,b 张 彬 缪存铅 崔俭杰 (a 上海应用技术学院香料香精技术与工程学院 上海 200235) (b 上海应用技术学院上海香料研究所 上海 200232) 摘要 采用同时蒸馏萃取提取得到荷叶挥发油, 通过吹扫/捕集-热脱附法(PT-TD)对上述提取物中挥发性成分进行富 集, 以气质联用(GC/MS)进行定性检测, 同时与直接进样GC/MS 法分析的成分进行比较. 两种方法成功分离分析出有 机酸、酯、醛、醇、酚、烷烃、芳香烃、烯烃以及含氮、硫、氧杂原子的化合物等共计84 种成分, 其中PT-TD GC/MS 鉴定出63 种有机化合物, GC/MS 鉴定出41 种有机化合物, 有20 种成分共同检出. 对比分析表明: PT-TD GC/MS 的 吹扫/捕集-热脱附过程能富集各种组分, 相比 GC/MS 分析, 可以鉴定出微量成分及更多挥发性和半挥发性成分, 在精 油等挥发性成分的分析检测中使用优势明显. 关键词 吹扫/捕集-热脱附; 气质联用; 荷叶; 挥发油 Comparative Analysis of Volatile Oil from Lotus Leaves by Purge and Trap-thermal Desorption Gas Chromatography-mass Spectrometry Zhang, Yunbin*,a Miao, Cunqiana,b Cui, Jianjiea,b a ( School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200235) b ( Shanghai Research Institution of Fragrance and Flavor Industry, Shanghai Institute of Technology, Shanghai 200232) Abstract Volatile oils were extracted from lotus leaves using simultaneous distillation extraction (SDE). Volatile components in lotus-leaf extract were enriched through a purge and trap-thermal desorption (PT-TD) method, and analyzed qualitatively with gas chromatography-mass spectrometry (GC/MS), which were comparative by analyzed with the method of direct injection GC/MS simultaneously. Totally 84 organic compounds we