中晚期胰腺癌裸鼠模型的代谢组学研究电子与通信工程专业论文.docxVIP

3．2．3生物样本的一维1H 3．2．3生物样本的一维1H NMR谱采集 4l 3．2．4数据处理与分析 41 3．3结果与讨论 42 3．3．1胰腺癌模型成功建立 42 3．3．2血样一维1H NMR谱 ． 一44 3．3．3血样多元统计建模与分析 45 3．3．4组织一维1H NMR谱 53 3．3．5组织多元统计建模与分析 54 3．3．6血样与组织相关的代谢通道分析 62 3．4本章小结 ． ．．69 第四章总结与展望 ．75 4．1论文总结 75 4．2研究展望 77 附录 79 攻读硕士期间发表的论文 ．．83 致谢 ．．84 万方数据 1．1 1．1 Background and research status ofpancreatic cancer ． ． ．． ． ． ．．1 1．1．1 Introduction ofpancreatic cancer ．1 1．1．2 Common diagnostic methods for pancreatic cancer ．．1 1．1．3 Research status and development trend ofpancreatic cancer 2 1．2AbriefintroductiontoNMR-basedmetabolomics 3 1．3 Research contents and methods in this thesis ．．4 Chapter 2 Metabolic relations between human and different animal species by metabonomic techniques ． ．．1 1 2．1 Introduction 1 1 2．2 Matcrials mad tmthods 1 1 2．2．1 Animal selection and sample coUection ofbjological fluid ．．1 1 2．2．2 Preparation ofbiological fluid samples ．12 2．2．3 NMR detection ofbiological fluid samples ．12 2．2．4 Spectral processing and multivariate statistical analysis ．．13 2．3 Results mad analysis ．．15 2．3．I Comparison ofserum and urine metabolic profiles between experimental animals aIld hurmn 1 5 2．3．2 Metabolic variations between human and different animal species 17 2．3．3 Metabolic pathways involved in species variations ．30 2．4 Summary ．．34 Chapter 3 Metabonomic study on advanced pancreatic cancer in animal modet ．． ．．． ． 、 39 万方数据 3．1 3．1 Introduction 39 3．：!Mat℃rials and mcthods 39 3．2．1 Establishrmnt ofanimal model with advanced pancreatic cancer． ．． ．．．39 3．2．2 Biological sample preparation ． ． ．41 3．2．3 1D 1H NMR spectrum acquisition 41 3．2．4 Data processing and amlysis ． ．41 3．3 R鹤ulta and disemsion ．． 。． 。 ．． ． ． ．． ．． ．．．． ．． 42 3．3．1 Successful establishment ofanimal model with pancreatic cancer． ． ．．42 3．3．2 1H NMR metabolic profiles ofserum samples ． ． ． ． ．44 3．3．3 Multivariate statistical n的deli