γ氨基丁酸gaba调控盐胁迫下玉米种子萌发和幼苗生长的机制作物学作物栽培学与耕作学专业论文.docxVIP

目录3．7．6基因差异表达聚类分析 目录 3．7．6基因差异表达聚类分析 ．68 3．7．7差异基因维恩图 69 3．7．8差异表达基因GO富集分析 ．．70 3．7．9差异表达基因PATHWAY富集分析 ．71 3．7．10差异表达基因的筛选及功能注释 7l 4讨论 ．．73 4．1 GABA调控NaCI胁迫下种子萌发 ．．73 4．2 GABA调控NaCI胁迫下幼苗形态建成 73 4．3 GABA调控NaCI胁迫下幼苗保护酶活性 74 4．4 GABA调控NaCI胁迫下幼苗氮代谢相关酶活性 75 4。5 GABA调控NaCI胁迫下幼苗内源激素含量 77 4．6 GABA调控NaCI胁迫下叶片光合系统 78 4．7转录组测序 ．．79 5结论 ．．80 弱!谢 ．．83 参考文献 ．84 攻渎博士学位期间发表的学术论文 99 万方数据 CONTENTSCONTENTS CONTENTS CONTENTS Abstract in Chinese ·I Abstract in English ．III 1 IntrOduction 1 1．1 Purpose and significance J 1．2 Research progress ·1 1．2．1 Effect of saIt stress maize ．．2 1．2．2 Resistance mechanisms ofmaize salt stress ．．4 1．2．3 Management of salt stress ·8 1．2．4 Introduction ofGABA ．12 1．2．5 Metabolic pathways ofGABA in plants ··13 1．2．6 Metabolic pathways ofGABA in plants ．．13 1．2．7 Technology roadmap lO 2 Materials and methods ··17 2．1 Materials 17 2．2 Experimental design ··1 2．2．1 The design of seed germination ··l， 2．2．2 The design of seedling growth ·1， 8 2．2．3 The design of RNA．seq ··1 2．3 Indicators and methods 18 2．3．1 Seed gemination 19 2．3．2 Seedling morphology and dry and flesh weight ··19 2．3．3 0xidative damage and antioxidant enzymes ··20 2．3．4 Photosynthetic system ·20 2．3．5 Nitrogen metabolism 20 2．3．6 Endogenous hormones ·21 2．3．7 RNA．seq 21 2．4 Data analysis tools ·22 3 ResuIts and analysis ．．- -24 3．1 Effect of various concentrations NaCI seed germination and seedling growth ·24 3．1．1 Seed germination 24 3．1．2 Seedling growth ·20 3．2 Effect of various concentrations GABA seed germination and seedling growth under NaCl stress ·29 3．2．1 Seed gennination 29 3．2．2 Coleoptile and radicle length 29 万方数据 东北农业大学农学博士学位论文3．2．3 东北农业大学农学博士学位论文 3．2．3 amylase activity ．．30 3．2．4 Plant height ．．3 l 3．2．5 Dry and fresh weight of leaf and stem ．32 3．2．6 Root growth ．33 3．3 Regulation of exogenous GABA oxidative damage and antioxidant enzyme of maize leaf under NaCl stress 37 3．3．1 MDA content 37 3．：；．2 Superoxide anion content ．．3{； 3．3．3 Histochemical loc