不同地区苏云金芽胞杆菌vip3aα基因的鉴定及杀虫活性分析生物学生物化学与分子生物学专业论文.docxVIP

C(扑iTE》iTSCoNTENTS C(扑iTE》iTS CoNTENTS Abstract in Chinese ． ．．I Abstract in English ． ．．Ill 1．Introduction ．．1 l。l Bacillus thuringiensis ．．1 1．2 Insecticidal Crystal Proteins 2 1．2．1 Type of Insecticidal Crystal Proteins 2 1．2．2 Mechanism mode of ICPs 2 1．2．3 Application ofICPs ．4 1．3 Vegetative Insecticidal Proteins 5 1．3．1 Insecticidal aetivites of VIPs ．5 1．：；．2 Mechanism mode ofVIPs ． ． 7 1．3．3 Application prospect ofVIPs ．11 1．4 nle methods to identify gene of insecticidal protein 12 1．4．1 PCR．IU?LP ．12 1．4．2 Molecular hybridization ofnucleic acid ．12 1．4．3 Identification ofprotein．．．． ．． ．．． ．． ．．．． ． ．． ．．．．．． ． ．．． 13 1．4．4 HRM ～1 3 1．4．5 PCR．HTS 14 1．5 The improtant agriculture pest 14 1．!；．1 Spodoptera exigua ．14 1．5．2 Helicoverpa armigera ．．14 1．6 Purpose and Significancy 15 2．Materials and Methods ．．1 6 2．1 Matedals ． ． ．16 2．1．1 Strains and plasmids ． ． ． ． ．．1 6 2．1．2 Enzyme and reagent ．1 6 2．1．3 Culture medium 。 ． ． ．。17 2．1．4 Antibiotics ．．1 7 2．1．5 Commonly used solution and buffer solution ．．1 7 2．1．6 Agriculture pest ． ． 21 2．1．7 Instruments ． ． ．21 2．1．8 Standard molecular weight ． ． 22 2．2 Methods ．22 2．2．1 Isolation of Bacillus thuringiensis ．．22 2．2．2 Crystal microscope observation 23 III 万方数据 东北农业大学理学硕士学位论文2．2．3 东北农业大学理学硕士学位论文 2．2．3 Extraction of total DNA orBtstrains ．．23 2．2．4 Designation of primers ．． ． ． ．． ． ．．． ．．． ．23 2．2．5 PCR ．．． ． ． ．． ．．． ． ．．．． ． 23 2．2．6 Digestion system ． ． ．． ．． ．． ． 24 2．2．7 Agarose gel electrophoresis