白三叶人工种子制作技术研究园林植物与观赏园艺专业论文.docxVIP

根多且发达，生根率达到了90％。在不加任何激素的MS培养基中，生根速度慢，且根 根多且发达，生根率达到了90％。在不加任何激素的MS培养基中，生根速度慢，且根 系瘦弱、侧根少。 (4)选用垂盆草2-4mm带腋芽茎段作为包埋外植体，采用滴珠法，以MS+4％海 藻酸钠+lmg／L 6-BA+0．1mg／L GA3+0．5mg／LNAA为人工胚乳，与2％CaCl2水溶液发生 离子交换15min后获得的垂盆草人工种子有弹性、呈球形；播种在MS培养基中20d萌 发率95％，30d生根率90％，在培养皿中催芽成苗率可达60％；此为制备垂盆草人工种 子的适宜方法。 (5)选择胚性愈伤组织再分化的丛生苗的带腋芽茎段作为人工种胚，以4％海藻酸 钠+O．5mg／L 6-BA+0．1mg／L GA3+0．6mg／L IBA做人工胚乳包埋，与2％CACl2水溶液离子 交换15min，形成硬度适中且富有弹性的球形人工种子，自三叶人工种子萌发率超过 70％，生根率超过60％。人工胍乳中添加活性炭抑制了人工种子的萌发。 关键字：白三叶；垂盆草；胚性愈伤；石蜡切片；再分化；包埋；人工种子 万方数据 Study Study on the Preparation Technique on Artificial Seeds of Trifofium repens Linn Abstract Trifolium repens L．is a member of genus Trifolium，Leguminosae，which is also known as white clover．This plant is one of high—yield and quality forage．Because of the advantages of strong aggressiveness and extensive management，long green period，and good omamental effect，it is often used as the slope greening plants．The application of Z repens is limited to a certain extent because of its seedling narrow ecological adaptation，weak drought tolerance， and not salinity．Some achievements have been made in the research on Z repens in transgenic，antiviral resistance，physiological stress etc．Our research team has got the new transgenic materials of Z repens with drought and salinity tolerance better than the common variety‘HAIFA．This study established the efficient tissue culture system of Z repens L．from the embryogenesis callus re-dedifferentiation．The artificial seed production technology of Z repens WaS researched．This study also lays the theoretical foundation for the preservation and propagation of new materials of transgenic z repens，and provides technical support for using artificial seeds of drought resistant and salt tolerant transgenic Z repens in soil spray sowing to green the slope．The main results obtained are as follows。 (1)Callus was induced from stem，shoot tip，leaf and petiole as explants．The results showed that the callus from petiole grew fast with loose texture．The callus inducti