富自体CGF纤维蛋白膜对体外培养人牙髓干细胞生物学 特性的影响-口腔临床医学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要···········································································································1 英文摘要···········································································································4 英文缩写··········································································································8 研究论文 富自体 CGF 纤维蛋白膜对体外培养人牙髓干细胞生物学特性 的影响 前言··········································································································9 材料与方法····························································································10 结果··········································································································16 附图··········································································································18 附表··········································································································23 讨论··········································································································24 结论··········································································································28 参考文献·······························································································29 综述 牙髓干细胞及富自体 CGF 纤维蛋白的研究进展 34 致谢················································································································48 个人简历··········································································································49 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 富自体 CGF 纤维蛋白膜对体外培养人牙髓干细胞生物学特性的 影响 摘 要 牙髓干细胞（dental pulp stem cells, DPSCs）是一类具有自我更新能力 和多向分化潜能的成体干细胞，其在一定条件下能够向特定的细胞类型分 化，有作为种子细胞应用于组织工程，达到修复和重建牙髓组织及形成牙 髓-牙本质复合体的潜能。 富自体浓缩生长因子（Concentrated Growth Factors, CGF）纤维蛋白 被认为是第三代血小板浓缩物，其通过不间断差速离心自体静脉血制备而 成。CGF 中含有多种生长因子，已有研究证实其对骨髓间充质干细胞 （mesenchymal stem cells, MSCs）的增殖及矿化具有促进作用。 目的：采用酶消化法体外分离培养、鉴定人牙髓干细胞（human dental pulp stem cells, HDPSCs），分别通过 CCK-8 法及碱性磷酸酶定量试剂盒检 测不同浓度富 CGF 纤