登革病毒非编码3’亚基因组RNA的鉴定与功能分析-微生物学专业论文.docxVIP

目 目 录 英文缩略词表 英文缩略词表 PAGE PAGE 3 PAGE PAGE 10 目 录 HYPERLINK \l _bookmark0 英文缩略词表············································································································· 4 HYPERLINK \l _bookmark1 中文摘要····················································································································· 5 HYPERLINK \l _bookmark2 Abstract 8 HYPERLINK \l _bookmark3 前 言······················································································································11 HYPERLINK \l _bookmark4 第一部分 登革病毒非编码 3’亚基因组 RNA 的鉴定 ···········································13 HYPERLINK \l _bookmark5 第二部分 登革病毒非编码 3’亚基因组 RNA 产生机制的分析 ···························25 HYPERLINK \l _bookmark6 第三部分 登革病毒非编码 3’亚基因组 RNA 的功能研究 ···································38 HYPERLINK \l _bookmark7 总 结····················································································································54 HYPERLINK \l _bookmark8 参考文献····················································································································55 附 录 一 登革 1 型病毒广州 06 株基因组全长感染性克隆的构建与鉴定…...58 附 录 二 登革病毒单轮感染颗粒的制备与鉴定(摘要)………………………...68 综 述 虫媒黄病毒基因组 RNA 元件在病毒复制中的调控功能…………...72 论 文 Identi?cation and characterization of small sub-genomic RNAs in dengue 1–4 virus- infected cell cultures and tissues……………………79 个人简历…………………………………………………………..……………….85 致 谢………………………………………………………………..………….86 英文缩略词表 英文缩写 英文全称 中文全称 Amp ampicillin 氨苄青霉素 bp base pairs 碱基对 cDNA complementary DNA 互补 DNA CPE cyto-pathetic effect 细胞病变 CS cyclization sequence 环化序列 DV dengue virus 登革病毒 DEPC diethyl pyrocarbonate 焦碳酸二乙酯 DMEM Dulbecco’s modified eagle medium DMEM 培养基 dNTP deoxyribonucleoside triphosphate 脱氧核糖核苷三磷酸 EDTA ethylenedia minetetraacetic acid 乙二胺四乙酸 FBS fetal bovine serum 胎牛血清 FITC fluorescein isothiocyanate 异硫氰酸荧光素 gRNA genomic RNA 基因组 RNA IPTG isopropylthio-β-D-galactoside 异丙基硫代-β-D 半乳糖苷 kD kilodalton 千道尔顿 mRNA messenger RNA 信使 RNA M.O.I multiplici