肝脏恶性肿瘤患者微波消融治疗后淋巴细胞亚群及细胞因子变化的临床研究-肿瘤学专业论文.docxVIP

万方数据 万方数据 Changes of lymphocyte subsets and cytokines in patients with liver malignant tumor after microwave ablation treatment Abstract Objective To investigate changes of liver malignant tumor patients in lymphocyte subsets and cytokines after microwave ablation treatment. Methods To collect peripheral blood of 45 liver malignant tumor patients within 1 weeks before microwave ablation and after ablation of 1 weeks, 1months.Regulatory T cells (Tregs), T lymphocyte (CD4+, CD8+), natural killer (NK) cell composition (%)were detected by flow cytometry. IL-2, IFN-γ, TNF-α, IL-12, IL-4, IL-6, IL-8 and IL-10 levels (pg/ml) were detected by flow cytometry and fluorescence tester. Results Compared with before microwave ablation,the ratio of CD8+ reduced significantly after 1 weeks and 1 months ablation （ P0.05 ） . The level of IL-12 rised significantly after 1 weeks and 1 months ablation（P0.05）. The level of IL-6 decreased significantly after 1 weeks and 1 months ablation（P0.01）. The level of IL-10 decreased significantly after 1 weeks and 1 months ablation（P0.05）.The proportion of NK cells increased significantly after 1 months ablation（P0.01）. Compared with after 1 weeks ablation, the level of IL-12 rised significantly after 1 months ablation（P0.05）. Conclusion Microwave ablation treatment can ease the local tumor suppression on immune function of organism, while promoting the Th2/Th1 offset, regulating the immune function disorders of patients with liver malignant tumor. Postgraduate student:Hai - Wen Zhang（Oncology） Directed by Prof. Xing - Jun Zhuang Keywords ： microwave ablation treatment ； liver malignant tumor ； lymphocyte subsets；cytokine；immunomodulation 目 录 引言········································································································································ 1 第 1 章 资料与方法·············································································································· 2 1.1 一般资料························