γ-干扰素对体外冲击波致输尿管损伤的保护作用研究中西医结合;临床专业论文.docxVIP

PAGE PAGE 10 0.05)，同时治疗组与药物对照组比较 p0.05 无统计学意义。 结 论： IFN-γ能够预防及治疗 ESWL 输尿管损伤及 ESWL 输尿管纤维 化的形成。 关键词：IFN-γ；体外冲击波；输尿管损伤；保护作用 The Study About Protective Function Of γ-Interferon On Extracorporeal Shock Wave Induced Injury Of Ureter Objective: This study was designed through animal experiments to observe the effect of γ-interferon on ureteral fibrosis after ureteral injury caused by shock waves and the change of smooth muscle contraction,then study the possible mechanism about the inhibition effect of γ-interferon on ureteral fibrosis . Methods: 64 New Zealand white rabbits were randomly divided into treatment group A (n=16): receiving injection of γ-interferon and ESWL, model group B (n=16): receiving normal saline and ESWL, drug control group C (n=16): only receiving injection of γ-interferon but not ESWL, sham group D(n=16): only being put Titanium clips onureter. A, C 2h group received preoperative intramuscular with IFN-γ (400 IU / kg), while B group received the same volume of saline. 4 rabits in each group were randomly chosen and put to death at 14th ,21th ,28th ,35th each day, the ureter specimen samples received routine pathological examination and score; the ureteral smooth muscle contractility was observed. Results: ESWL of ureteral injury in rabbits after the histological score to quantify the results shown by pathology: control group and the sham group after 7d shows epithelial cell degeneration, lamina propria and muscle layer edema, hemorrhage, inflammatory cell infiltration, 14d and 21d shows increased epithelial cell degeneration, and muscle within the lamina propria increased congestion and edema, and visible capillaries and fibrous tissue proliferation, 28d atrophy seen after epithelial cells, proliferation of smooth muscle cell degeneration and fibrosis, the outer membrane and surrounding tissue see a large number of adhesive tissue, inflammatory cell infiltration, some stenosis, old scars. Treatment group, pathological changes of the ureter afte