丹酚酸B对被动吸烟大鼠肝损伤保护作用.PDFVIP

第28 卷 第3 期 天津中医药大学学报 Vol．28 No．3 2009 年9 月 Journal of Tianjin University of Traditional Chinese Medicine Sep．2009 丹酚酸B 对被动吸烟大鼠肝损伤的保护作用 甘 萍，聂桂丽，李艾珊 摘要：[ 目的] 探讨丹酚酸B 对被动吸烟大鼠肝损伤的保护作用及其机制。 [方法] Wistar 大鼠，随机分为3 组， 即正常对照组、烟熏组、丹酚酸B 预防组。分别测定3 组大鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）及乳酸 脱氢酶（LDH）的含量，同时分析血清及肝组织中超氧化物歧化酶（SOD）的活性及丙二醛（MDA）含量的改变。[结 果] 烟熏组与正常对照组相比，大鼠血清中AST、ALT、LDH、MDA 及肝组织中MDA 含量均显著增加，SOD 活性 显著降低（P＜0．01）；经丹酚酸B 预处理后，与烟熏组相比，血清中AST、ALT、LDH 、MDA 含量及肝组织中MDA 含 量均降低，而SOD 活性增高（P＜0．05）。 [结论] 丹酚酸B 对被动吸烟大鼠肝损伤有一定的保护作用，其机制可能 主要与抗氧自由基的损伤有关。 丹酚酸B；被动吸烟；肝损伤 关键词： 中图分类号： 文献标识码： 文章编号： （ ） R285.5 A 1673－9043 2009 03－0130-03 Protection effect of salvianolic acids B against liver injury in rats with passive cigarette smoking GAN Ping, NIE Gui-li, LI Ai-shan (Tianjin High Special School of Medicine, Tianjin 300222, China) Abstract:[Objective] To discuss the protection effect and the mechanism of salvianolic acids B in the development of live injury in rats with passive cigarette smoking. [Methods] Wistar rats were randomly divided into 3 groups: normal group, smoking group and salvianolic acids B prevention group. The con- tent of serum ALT, AST and LDH in every group was assayed and the changing of MDAs content and the activity of SOD in serum and hepatic tissue were analyzed. [Results] Compared with the normal ’ group, the MDAs content in the hepatic tissue and AST, ALT, LDH and MDA s content in the serum increased obviously in smoking group. The activity of the SOD decreased (P 0.01). After treating