广西都安酿葡萄皮渣中化学成分的分离、活性研究及白藜芦醇的提纯工世分析-study on separation and activity of chemical constituents from grape skin residue of du an brewing in guangxi and purification of resveratrol.docx

广西师范学院硕士学位论文广西师范学院硕士学位论文IIII水相萃取法两种方法对白藜芦醇进行初步纯化，通过 HPLC 检测，经双水相萃取法纯化后白藜芦醇的含量 13.3μg/mL，远远高于有机溶剂萃取法的 2.342μg/mL。 关键词：酿酒葡萄皮渣；活性分析；化学成分；结构鉴定；白藜芦醇；提纯 PAGE PAGE IVAbstractGrape belongs to deciduous vine, which is one of the oldest plants on earth and also one of the earliest fruit trees human cultivated. China has a long history in planting grape. What’s more, grape wine drinking had been documented in Li Shizhen’s Compendium of Materia Medica. Vitis quinquangularis Rehd. in Guangxi grows naturally in a special geographical condition—karst limestone formations. It has a rich resources and significant research value. In the process of modern winemaking, the grape pomace is the waste material and does not make full use of its effective chemical components. In this thesis, the active chemical components in the grape pomace were analyzed, and the research on resveratrol’s purification process was conducted. The main contents are divided into the following aspects:In this paper, the grape pomace were soaked in 95% ethanol, then extracted from the ethanol extraction with petroleum ether, ethyl acetate, n-butanol, at the same time, the corresponding parts of the resulting were collected and divided the extract into four parts from small to large according to the polarity. The antioxidant activity test on hydroxyl radical, superoxide anion radical and DPPH radical were carried out. And it reflected antioxidant capacity of each polar part based on the radical scavenging being measured. Among them, the free radical scavenging of ethyl acetate and n-butanol are equivalent to Vc or BHT. This proved that ethyl acetate and n-butanol phase have a good antioxidant capacity. Then the tumor cell toxicity test separately on four polar parts with SGC-7901 (human gastric cancer cells), HT-29 (human colorectal adenocarcinoma cells), HeLa (human cervical cancer cells) and PC-3 (human prostate cancer) were carried out. This experiment reflects different anticancer activity of different polar