Yale Cell Sorting Biosafety Program -- Ben Fontes and Geoff ：耶鲁大学的细胞分选的生物安全程序——本特斯和杰夫.pptVIP

* * Trade-off not having an analyzer in a hood, increased aerosol production created by sorter * * * * * * * * * * * * * * * * * * Magnetic columns may also be a valid approach in some instances * * * Glo-germ verse YP particles Size Clean-up Preparation Clumping, KI testing, new two color bead testing * * * * * Cell Analyzers: Cell Sorters: Pressures: 3-6 psi 20-70+ psi Aerosol Containment: Usually none Frequently AMS/Hoods Samples (at Yale): Always fixed Usually non-fixed What are the potential risks and hazards associated with flow cytometers and cell sorters ? Flow Cytometers/Analyzers: Greatest potential hazard or risk associated in running a cytometer: The users 1) Train every user 2) Establish clearly stated rules “But, I’ve been doing flow for years at XXXXX.” Why are you here? Can anything really go wrong with a sorter? The Bad News: Cell sorting is a procedure that intentionally creates droplets and aerosols. 80% of laboratory acquired infections are from an unknown route of exposure, but the majority are likely transmitted via airborne exposure. (Sulkin Pike 1951, Pike 1979) The Good News: There are currently no laboratory acquired infections directly attributed to the process of cell sorting. (Holmes, Perfetto; Cyto 2010) The Other Bad News: Manufacturer trainings place little to no emphasis on bio-safety and the potential risks associated with cell sorting and cell analyzers. So where do you start? Framework for RA/RM Risk Assessment Risk Management Pathogen Practices (good work practices) Procedures Protective equipment (clothing and equipment) Personnel Place (facility design) Pathogen – Initial Risk Assessment Principal Investigator- Risk Group Classification- BMBL Biosafety in Microbiological and Biomedical Laboratories (BMBL), Current Edition (/biosafety/publications/bmbl5/) Public Health Agency of Canada – Pathogen Safety Data Sheets and Risk Assessment