The Role of tRNA Modification Systemsin the Cellular StresstRNA修饰系统在细胞应激的作用.docVIP

下载本文档

3
0
约4.99万字
约 46页
2018-06-19 发布于福建
举报
版权申诉

The Role of tRNA Modification Systemsin the Cellular StresstRNA修饰系统在细胞应激的作用.doc

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

The Role of tRNA Modification Systemsin the Cellular StresstRNA修饰系统在细胞应激的作用

The Role of tRNA Modification Systems in the Cellular Stress Response By Margaret Daly A Thesis Submitted to The University at Albany, State University of New York In Fulfillment of the Requirements for An Honors Biology Bachelor of Science Degree College of Arts and Science Department of Biology 2009 Abstract Background Transfer RNA(tRNA) is a small chain of nucleotides that participates in protein synthesis by pairing its anticodon with an mRNA codon and transferring an amino acid to a growing polypeptide chain. tRNA methyltransferases are a group of enzymes that can modify nucleosides in or around the anticodon, as well as at other parts of the tRNA. Recently, some of these modifications have been reported to enhance the translation of proteins that help the cell respond to and/or repair DNA damage. We hypothesize that the modifications catalyzed by some of the tRNA methyltransferases (Trms) stabilize the interaction between the mRNA codon and the tRNA anticodon thus enhancing the translation of transcripts with specific codon usage patterns. This enhanced translation may increase the levels of certain proteins necessary for the cell to respond to stress. Furthermore, we predict that their activity will dictate cellular responses to environmental carcinogens and chemotherapeutics. Results We exposed yeast gene deletion strains to carcinogens that cause cellular stress such as DNA damage, protein stress, and oxidative stress. Thus far, the effects of methyl methanesulfonate, tunicamycin, paramomycin, bleomycin, hygromycin, rapamycin, and hydrogen peroxide have been tested on 13 trm knockout strains. The results suggest that three of the tRNA methyltransferases may have a larger role in the stress response than the other ten trms. In particular, those tRNA methyltransferases that act to modify tRNA at the anticodon, or in close proximity to the anticodon, are the most important tRNA methyltransferases involved in stress responses. Trm4 and Trm5 seemed to