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PowerPoint Presentation Writing in theSciences AbstractsPowerPoint演示文稿在科学文摘的写作
Writing in the Sciences: Abstracts Kristina Wilson Some slides adapted from Daniel Martinez Science Writing - Logic Readers will often read non-linearly Read the abstract, scan the references Read the introduction and conclusion Scan tables and/or figures Read the discussion, methods and results last Abstract Overview of what you did Informative Descriptive Outline*: Problem. Key topic. Method. State your main approach to solving the problem. Results. Provide one or two key results. Conclusion. Note your main conclusion. Editing Abstract Content ID the four parts of an Abstract Remove unnecessary parts Add missing information We’ll address style and grammar in the next section “In the Abstract”: A long-winded (273 word) Abstract from Nature 455, 1138-1142 (23 October 2008) Metals are needed by at least one-quarter of all proteins1, 2. Although metallochaperones3, 4, 5, 6, 7, 8 insert the correct metal into some proteins, they have not been found for the vast majority, and the view is that most metalloproteins acquire their metals directly from cellular pools. However, some metals form more stable complexes with proteins than do others. For instance, as described in the Irving–Williams series9, Cu2+ and Zn2+ typically form more stable complexes than Mn2+. Thus it is unclear what cellular mechanisms manage metal acquisition by most nascent proteins. To investigate this question, we identified the most abundant Cu2+-protein, CucA (Cu2+-cupin A), and the most abundant Mn2+-protein, MncA (Mn2+-cupin A), in the periplasm of the cyanobacterium Synechocystis PCC 6803. Each of these newly identified proteins binds its respective metal via identical ligands within a cupin fold. Consistent with the Irving–Williams series, MncA only binds Mn2+ after folding in solutions containing at least a 104 times molar excess of Mn2+ over Cu2+ or Zn2+. However once MncA has bound Mn2+, the metal does not exchange with Cu2+. MncA and CucA have signal peptides for different export
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