Benzene, toluene, ethylbenzene ad mxylene are 苯，甲苯，乙苯和m二甲苯是.docVIP

Differential gene expression profiling in human promyelocytic leukemia cells treated with benzene and ethylbenzene Sailendra Nath Sarma a,b, Youn-Jung Kim a Jae-Chun Ryu a,b,* a Cellular and Molecular Toxicology Laboratory, Korea Institute of Science Technology, P.O. Box 131, Cheongryang, Seoul, 130-650, Korea b Department of Biopotency/ Toxicology Evaluation, University of Science Technology, Daejeon, Korea Tel: +82-2-958-5070 Fax: +82-2-958-5059 E-mail: ryujc@kist.re.kr *Correspondences and requests for materials should be addressed to J.-C. Ryu (ryujc@kist.re.kr) Abstract Benzene and ethylbenzene (BE), the volatile organic compounds (VOCs) are common constituents of cleaning and degreasing agents, paints, pesticides, personal care products, gasoline and solvents. VOCs are evaporated at room temperature and most of them exhibit acute and chronic toxicity to human. Chronic exposure of benzene is responsible for myeloid leukemia and also ethylbenzene is also recognized as a possible carcinogen. To evaluate the BE effect on human, whole human genome 35 K oligonucleotide microarray were screened for the identification of the differential expression profiling. We identified 280 up-regulated and 201 down-regulated genes changed by more than 1.5 fold by BE exposure. Functional analysis was carried out by using DAVID bioinformatics software. Clustering of these differentially expressed genes were associated with immune response, cytokine-cytokine receptor interaction, toll-like signaling pathway, small cell lung cancer, immune response, apoptosis, p53 signaling pathway and MAPKKK cascade possibly constituting alternative or subordinate pathways of hematotoxicity and immune toxicity. Gene ontology analysis methods including biological process, cellular components, molecular function and KEGG pathway thus provide a fundamental basis of the molecular pathways through VOCs exposure in human lymphoma cells. This may provides a valuable information to do further analysis to