甘蔗RO16的转基因研究.docVIP

甘蔗ROC16的转基因研究 Gene transfer to sugarcane ROC16 摘 要甘蔗(Saccharum officenarum L.)是我国最主要的糖类经济作物，其生产率的大小将直接影响到蔗农的收入和国民经济的稳定发展。但是在实际的甘蔗生产过程中，各种病虫害的侵害将大大降低甘蔗的产量和品质。在众多的病虫害中，常见的甘蔗害虫是甘蔗螟虫（Piatraea Saacharalis F.）也是最主要的虫害。本研究在于将修饰的豇豆胰蛋白酶抑制剂基因sck）及基因Bt）导入甘蔗ROC16种质中。探索获得了农杆菌介导ROC16转基因研究的相关科学数据：诱导甘蔗ROC16愈伤组织的最佳2，4-D浓度为2.5 mg/L（诱导率为91.7%）介于ROC16愈伤组织会因2，4-D浓度过高而不易诱导芽分化，在实际的愈伤诱导中，采用了1 mg/L（诱导率为72.9%）。采用2 mg/L 6-BA+1 mg/L KT+200 mg/L car进行芽的高浓度培养基中诱导刺激分化15天，再转入0.5 mg/L 6-BA+0.25 mg/L KT+200 mg/L car的低浓度培养基中15天，最后转入1 mg/L 6-BA+0.5 mg/L KT+200 mg/L car培养基中持续分化，可解决ROC16在常规浓度下（1 mg/L 6-BA+0.5 mg/L KT+200 mg/L car）难以诱导芽分化问题。 关键词：甘蔗；ROC16；农杆菌；转基因；sck基因；Bt基因 ABSTRACT The sugar cane is the most main carbohydrate industrial crop in my country, Its productivity will directly affect the size of national economic income/stable development , But in actual sugarcane production process, various insect violations will greatly reduce the sugarcane yield and quality, In many of the insect pest, common cane cane is stemborer and one of the most important pests. This research is to modify cowpea trypsin inhibitor SCK gene and gene ROC16 sugarcane Bt germplasm.The exploration obtained the agricultural bacillus to lie between leads the ROC16 transgene research the related scientific data: Induction sugar cane ROC16 injuries the organization the best 2,4-D density is 2.5mg/L (inductivity is 91.7%), Roc16 between callus because 2,4-d high concentration and easily induced bud points actual injuries in the induction, has used 1mg/L (inductivity is 72.9%). Using 2mg/L 6 - BA + 1mg/L KT/car on 200mg + high concentrations of bud differentiation medium induced stimulus for 15 days, Again into 0.5 mg/L 6 - BA + 0.25 mg/L KT 200mg + / car of low concentration medium for 15 days, Finally 1mg/L to 6 - BA + 0.5 mg/L KT 200mg + / car medium continuous differentiation. ROC16 can solve in conventional concentration (1mg/L 6 - BA + 0.5 mg/L KT/car) 200mg + to induce bud differentiation question.But after ROC16 genetic tr