氨溴索增强左氧氟沙星在铜绿假单胞菌生物被膜形成时杀菌作用.docVIP

氨溴索增强左氧氟沙星在铜绿假单胞菌生物被膜形成时杀菌作用 　　【摘要】目的 观察盐酸氨溴索（AMB）对铜绿假单胞菌（P.a）的生物被摸（BF）破坏清除作用及其与左氧氟沙星（LFX）联合后对BF内P.a的影响。方法 分别建立PAO1的3 d和7 d体外BF模型，随机（随机数字法）分为空白对照组、AMB组，AMB+LFX组。AMB质量浓度分别为256 μg/ml和512 μg/ml。于体外早期和成熟BF模型形成后，分别加入不同质量浓度的AMB，作用24 h后，连续稀释法行载体表面BF内P.a活菌计数，SEM观察载体表面BF形态学变化； 采用单因素方差分析法（One-Way ANOVA）进行计量资料的统计分析。结果 无论是早期BF还是成熟期BF，药物作用24 h后SEM观察，AMB组载体表面BF较空白对照组明显减少，并且随着AMB质量浓度的增加，载体表面BF呈减少趋势。无论是早期BF还是成熟期BF，1 μg/ml的 LFX组可以减少BF内细菌（P 　　【关键词】铜绿假单胞菌；生物被膜；盐酸氨溴索；左氧氟沙星；杀菌作用 　　Ambroxol reinforce the bactericidal effect of levofloxacin on the Pseudomonas aeruginosa biofilm KONG Jin-liang， CAI Shuang-qi， CHEN Yi-qiang， YAN Ping， ZHANG Dong-wei， JIAN Li-juan，WU Hai-ying. Department of Respiratory Medicine， The First Affiliated Hospital of Guangxi Medical University， Nanning 530021，China 　　Corresponding author：CHEN Yi-qiang，Email：kjl071@163.com 　　【Abstract】Objective To observe the destructive and scavenging effect of ambroxol（AMB）on the biofilm（BF） of Pseudomonas aeruginosa （P.a）. To evaluate the synergistically bactericidal effect of AMB along with levofloxacin （LFX）on BF of P.a.Methods The early model （cultured for 3 d） and mature model （cultured for 7 d） of P.a wild strain （PAO1） BF were established， in vitro， respectively. The models were randomly（random number）divided into control group， AMB group and AMB+LFX group. The concentrations of AMB were 256 μg/ml and 512 μg/ml， respectively. When the early BF model and mature BF model were made， different concentrations of AMB were added in AMB group and AMB+LFX （1 μg/ml） was added in AMB+LFX group. The number of viable P.a on the BF carrier was counted with the continuous dilution method 24 h after AMB or/and LFX added. Then， the BF morphological changes on the carrier surface were observed by using scanning electron microscopy （SEM）. Measured data were analyzed with single factor analysis of variance（One-Way ANOVA）. Results Both in the early BF model and in the mature BF model， the SEM examination showed that the BF in AMB group was s