一点红化学成分分离 分析及抗氧化活性分析-analysis of chemical constituents and antioxidant activity of emilia sonchifolia.docxVIP

阳性对照品Vc相当，综合各化合物和一点红乙酸乙酯部位的体外抗氧化实验结果，丁香酸和原儿茶酸可能是一点红乙酸乙酯萃取部位的主要抗氧化活性成分。关键词：一点红HPLC化学成分含量测定抗氧化活性STUDIESONTHECHEMICALCONSTIUENTSANDANTIOXIDANTACTIVITYOFEMILIASONCHIFOLIAABSTRACTThisarticledealedwiththestudyonEmiliasonchifoliawhicharecompositae,includingchemicalcompositionresearch,quantitativeanalysisandantioxidantacitivityofchemicalcomposition.TheobjectiveofthisarticleistoisolateandidentifychemicalcompositionfromEmiliasonchifoliausedtotraditionalChinesemedicine,andthiswilllaythefoundationforthefurtherdevelopmentofEmiliasonchifoliaformedicine.Variouscolumnchromatographictechniqueswereusedfortheisolationandpurification.FivecompoundswereseparatedfromthepartsofethylacetateextractofEmiliasonchifolia.Theirphysical-chemicalpropertiesandspectraldatadeterminedtoelucidatethestructures.Theirstructureswereprotocatechuicacid,p-hydroxybenzoicacid,p-hydoxyphenylacticacid,syringicacid,p-coumaricacid.Allthiscompoundswereisolatedfromthismedicinalmaterialsforthefirsttimeexceptp-hydroxybenzoicacid.Establishareversed-phasehighliquidchromatographic(RP-HPLC)methodforsimultaneousdeterminationoffivephenolicacidcompound,includingprotocatechuicacid,p-hydroxybenzoicacid,p-hydoxyphenylacticacid,syringicacid,p-coumaricacid.ThemethodconsulttheresearchoftheHPLCfingerprintofEmiliasonchifolia.ThecontentoffivephenolicacidcompoundweremeasuredbyRP-HPLCatthewavelengthof273nmandgradientelutedprogramwereusedandthemobilephasesystemwasmethanoland0.4%aceticacidsolution.Thecontentofprotocatechuicacid,p-hydroxybenzoicacid,p-hydoxyphenylacticacid,syringicacid,p-coumaricacidwere48.70?g/g,21.44?g/g,105.27?g/g,15.98?g/g,20.60?g/grespectively.TheRP-HPLCmethodfordeterminationthecontentofphenolicacidcompoundhadhighsensitivityandgoodreproducibility.StudyontheantioxidanteffectsoffivecompoundswhichwereseparatedfromthepartsofethylacetateextractofEmiliasonchifolia.ThedeterminationofantioxidantincludesDPPHradicalscavengingcapacity,·OHscavengingability,reduceingpower.Theresultssuggestthatprotocatechuicacidandsyringicacidwereon