免疫介导心脏疾病新策略课件.ppt

* * * * Cytokine expression was examined using RNase protection assay. The expression of IFN-γwas significantly suppressed in allografts treated with pioglitazone compared with controls. * * * Acknowledgement Tokyo Medical and Dental University Jun-ichi Suzuki Hisanori Kosuge Noritaka Koga Noriko Tamura Masahito Ogawa Osaka University Ryuichi Morishita Yasufumi Kaneda Kyushu University Kensuke Egashira Akita University Hiroshi Ito IL-10 IL-15 IL-6 IFN-γ Control Pioglitazone Suppression of Cytokines Expression in Allografts by Treatment with Pioglitazone RNase protection assay 5 days after transplantation Suppression of T cell Proliferation by Telmisartan in Mixed Lymphocyte Reaction 0 0.2 0.4 0.6 0.8 Optical Density * * *p0.05 R: responder (recipient) S: stimulator (donor) R R + Tel 10-5M R + S R + S + Tel 10-5M R + S + Tel 10-6M Tel: telmisartan Ultrasound + microbubbles increase efficacy of transfection Decoy Microbubble Ultrasound (+) 　　 (+)　 (+) (-)　　　(+)　 (+) (+)　　　(-) 　 (+) 100 200 p0.05 * FITC Positive Cells per Artery No Ultrasound Ultrasound (Suzuki J, Isobe M, J Vasc Res, 2006) Suppression of Neointimal Formation by NF-κB Decoy Antisense cdk2 ODN Antisense cdk2 ODN 100 100 50 50 0 0 * * * P 0.01 P 0.01 E2F Decoy E2F Decoy No ODN No ODN NF- k B Decoy NF- k B Decoy * (%) (%) 28 Days Minor mismatch (DBA to B10D2) Vascular remodeling after injury Transfection without viral vector Microbubbles with ultrasound * * * * * To investigate the effect of pioglitazone on acute rejection, we performed cardiac transplantation using C3H/He recipients and BALB/c donors. The donor hearts were transplanted in recipients heterotopically. Recipient mice were orally administrated with either control chow or chow containing pioglitazone from 1 day before cardiac transplantation. The survival of cardiac allografts was evaluated by daily palpation. * In total allomismatch combination, the surv