祛异康对子宫内膜异位症模型大鼠细胞凋亡因子bcl 2及bax表达的影响-effect of quyikang on the expression of bcl 2 and bax in endometriosis model rats.docxVIP

异康大、中、小各剂量组均能明显抑制异位症模型大鼠异位内膜的生长, 其治疗机理可能是通过提升促调亡因子bax及降低抑凋亡因子bcl - 2在异位内膜组织中的表达而 实现的。其中以大、中剂量组疗效可靠。关键词祛异康煎剂；子宫内膜异位症；Bax and bcl - 2；免疫组化；实验研究Eff ect of QuYi Kang on bax and bcl - 2 of endomet r i osi s i n r at s Abst r actObj ect i ve：To obser ve t he expr essi on of t he apopt osi s f act or bax and bcl - 2 i n t he endomet r i um of endomet r i osi smodel r at s t r eat ed wi t h t hr ee di ff er ent doses of QuYi Kang decoct i on（a pr epar at i on of t r adi t i onal Chi nese medi ci ne），and st udy endomet r i osi s i n t he pat hogenesi s and t he mechani sm of QuYi Kang t r eat i ng t he endomet r i osi s i n mol ecul e l el vel , att empt i ng t o pr ovi d obj ect i ve basi s f or accur at l y usi ng i n cl i ni c.Met hods: By usi ng aut opl ast i c t r anspl ant at i on t o devel op ani mal model of Endomet r i osi s , 60 heal t hy f emal e SD r at s at t he age of 60 days wer e r andoml y di vi ded i nt o si x gr oups:t he cont r olgr oup( A) ,t he modl e gr oup( B) , QuYi Kang l ow- dose gr oup( C) , QuYi Kang mi ddl e- dose gr oup( D) , QuYi Kang hi gh- dose gr oup( E)and Danazolgr oup （ F ） . The gr oup A was onl y swi t ched abdomen,t he ot hergr oups( B, C, E, D, F) wer e const r ct ed i nt o endomet r i osi s modelr at s. A and B gr oups wer e f ed wi t h 0. 9%Nacl . C, D and E gr oups wer e al l f ed wi t h QuYi Kang decoct i on( Gr oup C decoct i on was l ow- dose, Gr oup D decoct i on was mi ddl e- dose, Gr oup E decoct i on was hi gh- dose) . Gr oup F was f ed wi t h Danazol f l i ud. Medi ci ne wer e congt i nual l y gi ven t o t he r at s f or f our weeks. Al l r at s wer e ski l l ed at t he met aest r s st ages. Except t he r at s of gr oup A ,t he r at s of t he ot her gr oups wer e all ext r act ed t he eut opi c and ect opi c endomet r i um. Aft er obser vi ng t he change of anat omy,t he endomet r i um wer e embedded i n t he par aff i n wax. Sli ces whi ch wer e t aken f r om par aff i n wax