Flow Cytometry Tutorial ppt - STLCCedu Users Server Home：流式细胞术教程ppt stlccedu用户服务器的家庭.pdfVIP

Introduction to Flow Cytometry presented by: FlowFlow CytometryCytometry CoreCore FacilityFacility Biomedical Instrumentation Center Unifformedd Services University TopicsTopics CoveredCovered inin thisthis LectureLecture • WhatWhat isis flowflow cytometry?cytometry? • Flow cytometer instrumentation. •• TheThe useuse ofof fluorochromesfluorochromes inin flowflow cytometrycytometry. • Immunophenotyping. •• CompensationCompensation. • Data analysis and gating. • ClinicalClinical applicationsapplications. • Research applications. • EExamplle off an experiimentt usiing flflow cyttomettry. Flow Cyytometryy-- a.k.a. FACS • Flow cytometry is a technique used to measure thethe physicalphysical andand chemicalchemical propertiesproperties ofof cellscells oror cellular components. •• CellsCells areare measuredmeasured individuallyindividually, butbut inin largelarge numbers. •• SynonymousSynonymous withwith FACSFACS (fluorescence(fluorescence-activatedactivated cell sorter). • Also,Also, simplysimply referredreferred toto asas “Flow.Flow.” “Seeing” Cells • Microscopists visualize cells based on their morphology and staining characteristics. • Flow cytometrists measure cells based on similar characteristics. • Hence,, usingg flow cytometry, a cell can be “seen” both qualitatively and quantittitatitivelly. Historically… • In the clinical lab, mixed cell populations of the blood were evaluated manuallyy byy microscoppe. • In the 1950’s, the Coulter counter automated cell countingcounting basedbased onon size. Neutrophil Eosinophil