沙田柚钙调素结合蛋白(CaMBP)基因的鉴定和序列分析.pdfVIP

Open Journal of Nature Science 自然科学, 2017, 5(2), 203-210 Published Online May 2017 in Hans. /journal/ojns /10.12677/ojns.2017.52027 Identification and Sequence Analysis of a Calmodulin-Binding Protein Gene from Citrus Grandis var. Shatinyu Hort Qiong Luo, Hongni Tan, Shili Huang, Huaying Liu, Xinmin Qin* College of Life Science, Guangxi Normal University, Guilin Guangxi th th th Received: May 6 , 2017; accepted: May 20 , 2017; published: May 27 , 2017 Abstract In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. A calmo- dulin-binding protein gene sequence of Citrus grandis var. Shatinyu Hort was obtained though dif- ferential analysis method, and some characters of the calmodulin-binding protein were analyzed and predicted by bioinformatics method, including the composition of amino acid sequence, phy- sicochemical parameters, hydrophobicity, transmembrane domain, secondary structure and func- tion of protein etc. The results showed that the calmodulin-binding protein gene was 1616 bp in length with an open reading frame (ORF) of 1368 bp, encoding 455 amino acids with deduced mo- lecular weight of 50.11 KDa, and theoretical pI value of 5.33, and contained a calmodulin-binding proteins domain. Bioinformatics analysis showed that the calmodulin-binding protein was a hy- drophobic and unstable protein. The homology analysis of amino acid sequence indicated that the calmodulin-binding protein shared high homology with calmodulin-binding protein of Citrus cle- mentina (98%). This work provides a systemic sequence analysis of the calmodulin-binding pro- tein