急性髓细胞白血病中mir-155 ship1及akt的表达及临床意义-expression and clinical significance of mir - 155 ship 1 and akt in acute myeloid leukemia.docxVIP

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急性髓细胞白血病中mir-155 ship1及akt的表达及临床意义-expression and clinical significance of mir - 155 ship 1 and akt in acute myeloid leukemia.docx

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急性髓细胞白血病中mir-155 ship1及akt的表达及临床意义-expression and clinical significance of mir - 155 ship 1 and akt in acute myeloid leukemia

摘要4、SHIP1mRNA在AML患者骨髓单个核细胞中呈低表达。AKTmRNA在AML患者骨髓单个核细胞中表达水平无明显变化。5、AML初治组中miR-155与SHIP1mRNA表达及SHIP1mRNA与AKTmRNA表达均存在负相关。关键词：急性髓细胞白血病；miRNA；miR-155；SHIP1；AKT；PI3K/AKT；ABSTRACTObjective:ToinvestigatetheexpressionofmiR-155andSHIP1,AKTmRNAinthebonemarrowmononuclearcells(BMMCs)ofacutemyeloidleukemia(AML)patients,andanalyzetheclinicalsignificanceofmiR-155andSHIP1,AKTinpatientswithAML,andexplorepreliminarilytheroleofmiR-155andPI3K/AKTsignalingpathwayinthepathogenesisofAML,providingtheoreticalbasisforthetargettreatmentofmiR-155.Methods:Total80casesofbonemarrowsampleswerecollectedfromAMLpatients,ofwhichincluded46casesofnewdiagnosedpatients,34casesofpatientsincompleteremission;Thecontrolgroupin11cases,forpatientswithirondeficiencyanemia.Real-timefluorescentquantitativePCRmethodisusedtodetectrespectivelytheexpressionlevelofmiR-155,U6andSHIP1,AKT,GAPDHmRNAinbonemarrowsamplesfromAMLpatients.TherelativequantificationmethodwasusedtocalculatetherelativeexpressionlevelsofmiR-155andSHIP1,AKTmRNA,theformula2-ΔCt×102wasusedtocalculaterelativegeneexpressionvalues.SPSS17.0statisticalsoftwarewasusedtoanalyzetheexperimentaldata.Measurementdataarenon-normaldistribution,weredescribedbythemedian,usingMann-WhitneyUtest,Spearmancorrelationtestwasusedtoanalyzethecorrelation;thechi-squaretestwasusedforcountdata.Pvalue0.05wasconsideredstatisticallysignificant.Results:TherelativeexpressionofmiR-155inbonemarrowmononuclearcellfromAMLpatientsofthenewdiagnosedgroup4.2931(2.1642~8.8388)andtheremissiongroup3.6853(2.3697~5.2406)weresignificantlyhigherthanthecontrolgroup2.5190(0.9848~4.9360),Pvalueswererespectively0.001and0.004,thedifferenceswerestatisticallysignificant(P0.01);theexpressionlevelofmiR-155ofthenewdiagnosedgroupwasalsosignificantlyhigherthantheremissiongroup,thedifferenceswerestatisticallysignificant(P=0.022).TherelativeexpressionofmiR-155inbonemarrowmononuclearcellfromAMLpatientsofthemoderateprognosisgroup4.3587(2.3519~8.8388)andthepoorgroup5.8910(3.2193~7.9801)weresignificantlyhigherthanthefa