ang1 7mas通路对长期高脂喂养大鼠胰岛微循环及其功能的影响word格式论文.docxVIP

％[(6.72±0.39 vs 5.19±0.81 )mg?kg-1?min-1 ,P＜0.05]，胰岛素分泌曲线达峰时间前 移，急性胰岛素分泌反应（AIR）升高 55％（65.65±17.52 vs 42.25±14.39，P＜0.05）， 免疫荧光结果示β细胞内胰岛素表达量显著升高（P＜0.05），胰腺微循环血流量增 加 46％[(0.866±0.110 vs 0.595±0.062) ml·g-1·min-1，P＜0.05]，免疫荧光染色示胰 岛内 vWF 表达量增加 40％（相对浓度分别为 19.3±3.16 vs 13.8±2.21 P＜0.05），微 血管密度显著增加，胰岛 vWFmRNA 表达水平升高 35％（P＜0.05）；给予 MAS 受体阻断后，与 H1 组相比，H2 组葡萄糖输注率（GIR）降低 21％[(5.33±0.31 vs 6.72±0.39)mg?kg-1?min-1 ,P＜0.05],胰岛素分泌高峰延迟，急性胰岛 素分泌反应（AIR）下降 23％（50.60±14.39 vs 65.65±17.52，P＜0.05），β细胞内 胰岛素表达量显著降低（P＜0.05），胰腺微循环血流量降低 35％[(0.561±0.099 vs 0.866±0.110) ml·g-1·min-1，P＜0.05]，免疫荧光结果示胰岛内 vWF 表达量下降25％（相对浓度分别为 14.4±2.85 vs 19.3±3.16 P＜0.05），胰岛内 vWFmRNA 表达 水平降低 15％（P＜0.05）。结论长期高脂高热量喂养诱导的的胰岛素抵抗大鼠，胰腺微循环血流灌注轻度降 低，胰岛微循环已经出现失代偿表现：内皮细胞功能受损，微血管密度降低。Ang（1-7）通过其受体 MAS 结合可改善胰岛内皮功能及其介导的胰岛微循环，进而 改善长期高脂高热量饮食大鼠的胰岛功能。关键词血管紧张素(1-7)；胰岛功能；胰岛素抵抗；胰岛内皮功能；胰岛微循环AbstractThe effect of Ang(1-7) / MAS pathway on pancreatic islets microcirculation and function in rats with long-term high-fat dietObjectiveThough the intervention of Ang (1-7) and the MAS receptor antagonist A-779 on long-term high-fat diet induced insulin resistant rats, we observed the effects of Ang(1-7)/MAS pathway on pancreatic islet endothelial cells, islet microcirculation and islet function, and explored its regulation on long-term high fat diet induced β-cell dysfunction and the associated mechanism.Methods80 Wistar male rats were fed with basal diet for 2 weeks , 20 of which were randomly selected as normal control group (NC group, n = 20), given normal diet. The remaining were fed with high-fat diet, randomly divided into high-fat group (H0, n = 20), Ang (1-7) intervention group (H1 group, n = 20), Ang (1-7) + A-779 intervention group (H2 group, n = 20) after 12 weeks, then each of them were treated with saline, Ang (1-7), Ang (1-7)+ A-779 though subcutaneous injection for 4 weeks. The intervention dose of Ang (1-7) and A-779 were both 300μg ? kg-1 ? d-1. Colored microspheres technique (sedimentation method) was used to estimate the