alvj hw2株的分离鉴定及其传播特性的分析word格式论文.docxVIP

correlation between antigen of albumen and antigen of cloacal swab.In short, the purification scheme be proposed, 50 days old, 70 days old, 120 days age are the key periods of the key monitoring antigen, antibody detection time should be in 80 day old to 90 days old of age.Key word：subgroup J Avian leucosis virus,isolation and identification,animal infectivemodelIII目录摘要.........................................................IAbstract....................................................II目录........................................................IV插图和附表清单..............................................VI主要符号表.................................................VII文献综述.....................................................1引言.........................................................71 材料与方法.................................................81.1 病料来源与细胞 ...........................................81.2 酶类、载体与主要试剂（盒） ...............................81.3 主要溶液与培养基的配制 ...................................81.3.1 DMEM 细胞基础培养液81.3.2 细胞培养生长液 .........................................81.3.3 细胞消化液.............................................81.3.4 谷氨酰胺溶液 ...........................................81.3.5 青链霉素溶液 ...........................................81.4 试验动物.................................................91.5 主要仪器.................................................91.6 ALV-J 的分离与鉴定91.6.1 病毒的分离 .............................................91.6.2 细胞培养液的 ELISA 检测91.6.3 细胞培养液的 PCR 检测101.6.4 gp85 序列的测定与分析111.6.4.1 gp85 基因的克隆111.5.4.2 gp85 基因的同源性及系统进化树分析121.7细胞培养物 TCID50 的测定131.8 试验动物的感染、分组与饲养 ..............................131.9 样品的采集与处理 ........................................141.9.1 采集泄殖腔拭子 ........................................141.9.2 血液采集 ..............................................141.9.3 精液的采集 ............................................14IV1.9 样品的检测..............................................141.9.1 病毒血症的检测 ........................................141.9.2 排毒的检测............................