09三羧酸循环.ppt

1. Acetyl in acetyl-S-CoA was oxydized: 化学氧化释放的自由能：874.9 kJ/mol 生物氧化释放的自由能：520.9 kJ/mol 用于ATP合成的自由能：354 kJ/mol 3.4 The energetic of the aerobic decomposition of glucose 葡萄糖有氧分解途径的能量计算 2. Hydrations in TCA-cycle: The third H2O entered in TCA cycle: Citryl-CoA+H20 succinyl-CoA + H2O Fumarate+H20 Citrate+ CoA-SH Succidate + CoA-SH Matalte H2O H2O 3.4 The energetic of the aerobic decomposition of glucose H2O 3. Dehydrogenations in oxydation of glucose: In EMP: C6H12O6 2×pyruvate + 4H In TCA cycle: 2×acetyl-CoA + 2× 3 H2O 16H + 4CO2 2 × CoA-SH So: C6H12O6 + 6H2O 24H + 6 CO2 6O2 12H2O C6H12O6 + 6O2 6H2O + 6 CO2 In formation of acetyl-CoA: 2×pyruvate 2 × CoA-SH 2×acetyl-CoA + 4H +2CO2 3.4 The energetic of the aerobic decomposition of glucose 4. Caculation of energy In EMP: ATP 4 - 2 Numbers of ATP 2NADH?H+ 2 × 2.5 In formation of acetyl-CoA: 2NADH?H+ 2 × 2.5 In TCA cycle: 3NADH?H+ × 2 3 × 2.5 × 2 FADH2 × 2 1 × 1.5 × 2 GTP × 2 1 × 1 × 2 Summery: 32 (→FADH2) (2 ×1.5 ) (30) 3.4 The energetic of the aerobic decomposition of glucose 1. All organic chemicals can be completely oxidized to CO2 and H2O via a cycled pathway; 所有的有机化合物都可以通过TCA环彻底降解为CO2 和 H2O ; 2. Plenty energy can be provided accompany the cycled pathway; 伴随着这一循环途径，大量的自由能被转换; 3.5 TCA环的生理意义 3. The intermediates in the cycle are important sources for biosynthetic precursors; TCA环中的中间代谢产物是重要的生物合成的前体 4. The cycle is an amphibolic pathway, and is a metabolic hinge of all biochemicals TCA环是一个分解合成无定向途径，是所有生物化合物的代谢枢纽。分解产能,合成前体. 3.5 TCA环的生理意义 They lack the a-keto—glutarate dehydrogenase; 厌氧菌缺乏a-酮戊二酸脱氢酶 Precursors are provided for the synthesis of amino acids, nucleotides, heme etc. 合成代谢的前体通过正向或逆向反应合成 Use an incomplete citric acid cycle as a source, not of energy, but o