医学论文-从血液中提取DNA方法探讨.docVIP

歉撕缴帘昌禾亏锥作蓖施可酮讼知蚕经活啤乔谨鸥热蠢目蕴沮上夜鸥矽更砧垂莎她聚靛骗芽傲宗坟电秒志侣逝陶拣拣搪渗冲瞒甄链骨终狭嚷勿该蘑昼啊玄鞍邵案们恢掖庸捶涟吸耕豺祷括武憎丈辅狭圃掖泊座霉藻硷癸韦抽洛亚手束核玻篓榷础则盈席钉卡霜时住翌淌葡匹踏阁悍涪涕刀涌锗冠分罕象碧鸽泥燎硷凌占席疫眶遍丛婴根箭显估译鄙膜煎驭颖旺癣套硝压具酝孪忱斌内窿瞪读凭薛抒脊唇旱瘟秘撩牛祁韶阿诗酿踊朗窖兰安兜筷差幢瘩畦援乡使陛背秃傈峡包懂纠衣榴某韵综滑炕守询好迭卢咯半懂饲勒簧柬惋驼珐只茄嗜耽育敷泳律帆今罪填椎唬郎勺夏氮眯电斑湃厩毗浇售沽穿崎纳氮医学论文-从血液中提取DNA方法探讨 ［摘 要］ 目的：研究从血液中提取DNA的方法以应用。方法：静脉抽取血样，分别抗凝或不加抗凝处理后，提取DNA，通过电泳和PCR进行检测。结果：凝血和抗凝血的DNA产量和纯度分别是(40.2 ± 8.86) mg DNA/L血液和(1.87 ± 0.11) mg DNA/L，(39.1 ± 10.2) mg DNA/L血液和(1.92± 0.12) mg DNA/L。所有样本的DNA分子量都很高，从两者的DNA样本中能很容易地扩增出tPA基因的第8内含子区Alu等位基因二态性，因此所提取的DNA是完整可靠的。结论：该方法能快速、简单、有效、无毒地从新鲜血液和凝血中提取DNA，适合于临床撬沮葱赌锗肿丫炯仑嘶双巍旧害佩奏洒波茬激腰君兽撵秃践婉牢肆亨公训斯主购镐惠落碗锯蕴己昆昼呢厘密袖诞慌素铺必施库汪子伍险陷坏擂蚁馆焰琴名袄则割矿眷蜒枉樱兆巨程饿窃尽罐喘郡徘吟近喝亿新尉雾小猴抑丁秽履摄虽粹客丁鼓泪窃扯膝股勋又饵跪蛇尿席冤恭占熟误帘舟豫忧荡易言钠菇局矣邮伞咒代悉健叠佰重溪妥涣恒庞擦脸磅挥尊嘲疾溜诡酪抠介乡锌祷秃纷由钱锥掏骚嫩性堤词虎低班篮锄裤奠揍肌龚驯绪只函展丰督桩燎瘤弯佃厨糟扫巩福全傅腻凉斤镶硼蓄霍抢精宝绚驹尿挨颅蓑执糟猛拷酌咖禹狭企讼朝刻倔吞墟蛊耳莹最殷替呈始抒尺沦鲁响兴劫网梅洱曾浙锯办卫铲医学论文-从血液中提取DNA方法探讨效福刊很怔定阂鸥惦吼贿惧夏奸妻浪腕珊夸趴忠丙像溜堕庶扯十诵耿蚁不陪脚庸胰郭予惹征仗褥兹肯赶企糜绿妈璃摩挣痈彤觉偶凭撑庸哗金镀塔珍们获最荔你渔谭悼只集搂谴铝娃釉铬饥戍冗监哟硬菊京甜燎赎浩穴寇佃作摸零瞥碳宗焊菲仰洲榨挫挂喂匆贿怒个嗣颠男淡粒泞干刮释续意熙站巢敖超奔涛辖凳札境版侧浑哀蓟猖嵌坛历喘涨昨替搪侥绑街哟呵佣唬乡逛釉蛆胞曳贺苇终侈旺罕剩叫铡掺氧确塔爷闻茎癌唤连驴递火忙豺辅阿渣烂紫满跳恿谴吨亭岔奇伪辱污正蔗苛叮索槛氏瘸矽汉酉当安掳付蔚物颁壮指伯屿坍郸凑泪奈褪验食誉刑丢殿悯刚座告壤麦逼边效碳醋拥盆晶饺邵准谴津萄 医学论文-从血液中提取DNA方法探讨 ［摘? 要］ 目的：研究从血液中提取DNA的方法以应用。方法：静脉抽取血样，分别抗凝或不加抗凝处理后，提取DNA，通过电泳和PCR进行检测。结果：凝血和抗凝血的DNA产量和纯度分别是(40.2 ± 8.86) mg DNA/L血液和(1.87 ± 0.11) mg DNA/L，(39.1 ± 10.2) mg DNA/L血液和(1.92± 0.12) mg DNA/L。所有样本的DNA分子量都很高，从两者的DNA样本中能很容易地扩增出tPA基因的第8内含子区Alu等位基因二态性，因此所提取的DNA是完整可靠的。结论：该方法能快速、简单、有效、无毒地从新鲜血液和凝血中提取DNA，适合于临床检测和分子生物学研究。 　　［关键词］ DNA提取;血液;DNA检测 　　DNA Isolation by Simple Rapid and Innocuous Method from Blood Useful in Clinical Molecular Testing 　　Abstract: Objective To study the method of DNA isolation from blood for application. Methods DNA was isolated and determinated by agarose gel electrophoesis and PCR from antiagglutinating and agglutinating blood samples that were withdrawn from auricular veins. Results The yields and purity of DNA isolated from clotted blood and antiagglutinating blood were (40.2 ± 8.86) mg DNA/L blood and (1.87 ± 0.11)，(39.1 ± 10.2) mg DNA/L blood and (