The Global control of FMD - Tools, ideas and ideals – Erice,….pdfVIP

TheGlobalcontrolofFMDTools,ideasandideals–Erice,Italy1417October2 8 Appendix 20 EXPRESSION OF FMDV CAPSID PROTEINS IN SILKWORM-BACULOVIRUS EXPRESSION SYSTEM AND ITS UTILIZATION AS AN EMPTY CAPSID VACCINE Z.Li1 2 1 3 3 2* 1 , Y.Yi ,X.Yin ,Zhidong.Zhang ,Y.Li ,ZhifangZhang andJ.Liu 1StateKeyLaboratoryofVeterinaryEtiologicalBiology,KeyLaboratoryofAnimalVirologyof MinistryofAgriculture,LanzhouVeterinaryResearchInstitute,ChineseAcademyofAgriculture Sciences,Lanzhou,Gansu,China 2BiotechnologyResearchInstitute,ChineseAcademyofAgriculturalSciences, Beijing,China 3InstituteforAnimalHealth,Pirbright,Woking,Surrey,GU240NF,UK ABSTRACT Introduction Footandmouth disease (FMD) is a highly contagious disease of livestock that causes severe economic loss in susceptible clovenhoofed animals. Although the traditional inactivated vaccine has been proved effective, it maylead to a new outbreak of FMDbecauseof either incomplete inactivation of FMDV or the escape of live virus from vaccine production workshop. Thus, it is urgenttodevelopanovelFMDVvaccinethatissafer,moreeffectiveandmoreeconomicalthan traditionalvaccines. Methodologyandprincipalfindings ArecombinantsilkwormbaculovirusBmP12A3CwhichcontainedtheintactP12Aand3Cprotease codingregionsofFMDVAsia1/HNK/CHA/05wasdeveloped.Indirectimmunofluorescencetestand sandwichELISAwereusedtoverifythatBmP12A3Ccouldexpressthetargetcassette.Expression productsfromsilkwormweredilutedto30foldsandusedasantigentoimmunizecattle.Specific antibody was induced in all vaccinated animals. After challenge with virulent homologou