Comparison of SDS gel capillary electrophoresis with microfluidic lab-on-a-chip technology to quantify relative amounts of 7S and 11S proteins from 20 soybean cultivars精品.pdfVIP

International Journal of Food Science and Technology 2009, 44, 2127–2134 2127 Original article Comparison of SDS gel capillary electrophoresis with microfluidic lab-on-a-chip technology to quantify relative amounts of 7S and 11S proteins from 20 soybean cultivars Vladimir Blazek Robert A. Caldwell* Faculty of Agriculture, Food and Natural Resources, University of Sydney, NSW 2006, Australia (Received 9 April 2009; Accepted in revised form 17 July 2009) Summary SDS gel capillary electrophoresis (Beckman–Coulter ProteomeLab) and the lab-on-a-chip technology (Agilent 2100 Bioanalyzer) were used to quantify the relative amount of 7S and 11S fractions in twenty different soybean cultivars. The better repeatability of the migration times and peak areas was achieved for the Bioanalyzer. Both lab-on-a-chip instrument and a traditional capillary gel electrophoresis were shown to be adequate for analysis of soy-based products. Integrating the area of peaks within a certain range of molecular weights was used to calculate the relative content of each protein subunit. Poor agreement in the classification in the protein subunit groups between the two instruments was observed. Therefore, the approach of visual identification taking into account both the variability in the position of the peaks and the detection of different number of peaks between the profiles was applied. This resulted in statistically significant correlation being observed between 11S⁄ 7S ratios determined by Bioanalyzer and ProteomeLab (R = 0.82). The reported differences in 11S and 7S content between the studies are li