pser40 tyrosine hydroxylase immunohistochemistry identifies the anatomical location of c1 neurons in rat rvlm that are a.pser40酪氨酸羟化酶免疫组织化学鉴定c1的解剖位置所激活精品.pdfVIP

Neuroscience 317 (2016) 162–172 pSer40 TYROSINE HYDROXYLASE IMMUNOHISTOCHEMISTRY IDENTIFIES THE ANATOMICAL LOCATION OF C1 NEURONS IN RAT RVLM THAT ARE ACTIVATED BY HYPOTENSION P. E. NEDOBOY, a S. MOHAMMED, a K. KAPOOR, a,b 30% and 45%, respectively) of the C1 area, but that following A. M. BHANDARE, a,b M. M. J. FARNHAM a AND unloading of the baroreceptors there is a marked increase in P. M. PILOWSKY a,c* activation of central (80%) and medial (90%) C1 neurons a The Heart Research Institute, 2042 New South Wales, Australia in the RVLM. Crown Copyright 2016 Published by Elsevier b Ltd. on behalf of IBRO. All rights reserved. Australian School of Advanced Medicine, Macquarie University, Sydney, 2109 New South Wales, Australia c Department of Physiology, University of Sydney, Sydney, 2006 New South Wales, Australia Key words: tyrosine hydroxylase, phosphorylated tyrosine hydroxylase, RVLM, C1 neurons, IgG subtype-specific Abstract—Identification of neurons, and their phenotype, monoclonal antibodies, baroreflex. that are activated in response to specific stimuli is a critical step in understanding how neural networks integrate inputs to produce specific outputs. Here, we developed novel INTRODUCTION mouse monoclonal antibodies of different IgG isotypes that are specific to tyrosine hydroxylase (TH), and to tyrosine The rostral ventrolateral medulla (RVLM) is a critical area hydroxylase activated at its