Intracellular applications of fluorescence correlation spectroscopy 荧光相关光谱内的应用精品.pdfVIP

583 Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience Sally A Kim and Petra Schwilleyz Based on time-averaging fluctuation analysis of small we must integrate our understanding of molecular events fluorescent molecular ensembles in equilibrium, fluorescence with the context of the cellular spatial organization. correlation spectroscopy has recently been applied to investigate processes in the intracellular milieu. The exquisite Current biochemical methods cannot account for the sensitivity of fluorescence correlation spectroscopy provides compartmentalized and interconnected nature of intra- access to a multitude of measurement parameters (rates of cellular biochemistry or the complexity and impact of the diffusion, local concentration, states of aggregation and native cytoplasmic milieu. Concepts of the intracellular molecular interactions) in real time with fast temporal and high environment have gradually evolved from the notion of a spatial resolution. The introduction of dual-color cross- viscous gel to the current view of an aqueous yet crowded correlation, imaging, two-photon excitation, and coincidence compartment [2]. In addition to the effect of compart- analysis coupled with fluorescence correlation spectroscopy has mental constraints, the presence of cytoskeletal elements expanded the utility of the technique to encompass a wide range and substantially higher total concentrations of macro- of promising applications in living cells that may provide molecules that do not necessarily participate in the reac- unprecedented insight into understanding the molecular