Cy3产品说明书.doc

PRODUCT AND SAFETY DATA SHEET Product Name: CY3 NHS ester CATALOG # OPA13101 Molecular Information: Properties: MWt: ~860 Color Form Dark red solid Solubility Soluble in DMF or DMSO Absorption/Emission 550nm/565nm (pH7 PBS) Extinction Coefficient ~150,000 Storage and handling: Store the original solid sample desiccated at -20 (C. Stock solution can be made in anhydrous DMF 1mg/100?L concentration. If stored at 4 (C, the stock solution should be stable for days. Protect the dye from light, especially in solution. application: Cy3 is an orange-red fluorescent dye that is particularly good for protein labeling, producing highly fluorescent protein conjugates. The dye is a superior alternative to the commonly used carboxytetramethylrhodamine (TAMRA) because it does not form aggregates and is very water-soluble. CY3 NHS ester is readily coupled to an amine group using the following recommended procedure. Typical Procedure for Labeling 1mg of IgG proteina Prepare 1mL of 1 M sodium bicarbonate (NaHCO3) solution by dissolving 84 mg of sodium bicarbonate in 1 mL deionized H2O. The resulting bicarbonate solution has a pH of ~8.3. If the proteinb is in a solid format, dissolve 1 mg of the protein in 0.5 mL 0.1M NaHCO3 buffer that is made from the 1 M NaHCO3 solution in step 1) by diluting 10 times with deionized H2O. If the protein is already in a solution and is greater than 2mg/mL, dilute the protein solution with PBS or 0.1 M sodium bicarbonate buffer to 2mg/mL. To 0.5 mL of 2mg/mL protein solution, add 50 ?L of the 1 M bicarbonate solution prepared in step 1. Prepare a stock solution of the dye by dissolving the dye in anhydrous DMF at 1mg/100?L concentration. The solution should be stable for a few days if kept at 4 (C. Regular DMF may be used for dissolving the dye, but the solution should be used within hours due to potential hydrolysis of the dye. The exact concentration of the dye can be determined by measuring the absorbance of an al