冷驯化条件下中缅树鼩脂肪组织切片的对比研究.docVIP

冷驯化条件下中缅树鼩脂肪组织切片的对比研究 孙舒然1，朱万龙1，陈金龙2，曹能1，高文荣1，左木林1，叶芳艳1，付家豪1，杨涛1，王政昆1( 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室，云南师范大学生命科学学院 ，云南 昆明 650500；2.昆明市海口林场，云南 昆明 650114） 摘要： 目的 探讨冷驯化不同时期中缅树鼩脂肪细胞形态变化，为冷驯化是否可以诱导白色脂肪组织中米色脂肪细胞(Beige Adipocytes)的产生提供形态学依据。方法 通过苏木素-伊红染色切片、免疫组织化学染色切片、免疫荧光染色切片进行对比。 结果 随着冷驯化时间延长，白色脂肪细胞和褐色脂肪细胞形态都明显变小，且褐色脂肪组织中解偶联蛋白1 (uncoupling protein 1, UCP1)表达增加，同时腹部皮下白色脂肪组织中可能已发生褐变，产生一些具有UCP1表达的浅褐色脂肪细胞，即米色脂肪细胞。结论 不同染色切片不仅能够清晰观察到脂肪细胞的形态改变，还能从抗体阳性颜色中观察出UCP1表达变化，为中缅树鼩脂肪组织研究提供基础性材料。 关键字：冷驯化；脂肪组织；苏木素-伊红切片；免疫组织化学切片；免疫荧光切片 The comparations of tree shrews (Tupaia belangeri) adipose tissue slices preliminary study under cold-acclimation SUN Shu-Ran1, ZHU Wang-Long1, CHEN Jing-Long2, CAO Neng1, GAO Weng-Rong1,ZUO Mu-Ling1, YE Fang-Yan1, FU Jia-Hao1, YANG Tao1, WANG Zheng-Kun1( (1.Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of University in Yunnan Province School of Life Science Yunnan Normal University Kunming 650500 China；2.Haikou Forest Farm of Kunming, Kunming 650114, China) Abstract: Objective The present study was aimed to understand the fat cells morphological changes during different periods under cold acclimation in tree shrews, whether the cold acclimation can induce cream-colored fat cells in white adipose tissue (Beige Adipocytes), and provide morphological basis. Methods The Hematoxylin-Eosin staining sections, Immunohistochemical staining sections and Immunofluorescence staining sections are compared. Results With the prolonged cold acclimation, the white fat cells and brown fat cells are decreased significantly, and the uncoupling protein1 expression in brown adipose tissues are increased. At the same time, abdominal subcutaneous white adipose tissue may have occurred browning, produce some shallow brown fat cells with UCP1 expression, namely the beige fat cells. Conclusions Different staining sections not only can clearly observe the morphological changes of fat cells, but also observe UCP1