Identifying and classifying functional small RNAs from pine：识别和分类功能的小分子RNA的松树.pptVIP

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Identifying and classifying functional small RNAs from pine：识别和分类功能的小分子RNA的松树.ppt

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Identifying and classifying functional small RNAs from pine：识别和分类功能的小分子RNA的松树

Identifying and classifying functional small RNAs from pine Ryan Morin BC Genome Sciences Centre (presenting research conducted in the lab of Dr. Peter Unrau) Small RNAs in the land plants MicroRNA Biogenesis MicroRNA (miRNA) transcripts can be mono- or polycistronic Each miRNA derives from its own hairpin foldback region of the primary miRNA transcript A number of processing steps are required to form the mature miRNA sequence The final mature miRNA is bound to a complex of proteins known as RISC MicroRNAs are post-transcriptional gene regulators MiRNAs are short (18-24nt) RNAs that are derived from larger precursor transcripts Mature miRNAs hybridize to near-complementary sites within their target messenger RNAs Messenger RNAs hybridized to microRNAs are enzymatically degraded, preventing translation siRNAs can be post- or pre-transcriptional gene regulators siRNA precursors are also processed by dicer (plants have multiple Dicer paralogs) Many active mature siRNAs are produced from the primary duplex Purification, adaptor ligation and amplification of small RNAs Rice and Pine sequences produced by 454 SBS technology 142,493 reads from pine, 11,436 from rice Small RNA sequence was extracted by identifying flanking adaptor sequences Random tag sequence was used to separate PCR-amplified constructs from multiple occurrences of the same small RNA species There were 58,466 unique sequences from pine and 8,615 from rice Most common sequence was observed 1168 times in pine and 89 times in rice Positional annotation of small RNAs and identification of conserved pine small RNAs Alignment of both of pine small RNA sequences to the rice genome reveals perfectly conserved pine small RNAs Pine sequences aligning to rRNA or tRNA genes in the rice genome are likely degradation products of pine rRNA/tRNA Sequences aligning within annotated regions were annotated as rRNA, tRNA, miRNA, repeat-derived siRNA etc. Positionally-annotated small RNAs show distinct trends in sequence