study of laser uncaging induced morphological alteration of rat cortical neurites using atomic force microscopy：激光解除引起的形态学改变大鼠皮层突起利用原子力显微镜研究.pdfVIP

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study of laser uncaging induced morphological alteration of rat cortical neurites using atomic force microscopy：激光解除引起的形态学改变大鼠皮层突起利用原子力显微镜研究.pdf

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study of laser uncaging induced morphological alteration of rat cortical neurites using atomic force microscopy：激光解除引起的形态学改变大鼠皮层突起利用原子力显微镜研究

Journal of Neuroscience Methods 253 (2015) 151–160 Contents lists available at ScienceDirect Journal of Neuroscience Methods j o u r n a l h o me p a g e: w w w. elsev /locate/jne umeth Basic neuroscience Study of laser uncaging induced morphological alteration of rat cortical neurites using atomic force microscopy Jian Tian a,b,1, Chunlong Tu a,b,1, Yitao Liang a,b,1, Jian Zhou a,b,1, Xuesong Ye a,b,∗ a Biosensor National Special Laboratory, Key Laboratory of BME of the Ministry of Education, Zhejiang University, Hangzhou 310027, PR China b Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China h i g h l i g h t s • A platform for combining laser uncaging stimulation with atomic force microscopy scan is set up for neuroscience researches. • Uncaging of glutamate at low frequency induces a calcium-related cortical neurite collapse. • The platform has the advantages of super resolution imaging, highly localized stimulation and convenient platform building. a r t i c l e i n f o a b s t r a c t Article history: Activity-dependent structural remodeling is an important aspect of neuronal plasticity. In the pre- Received 10 March 2015 vious researches, neuronal structure variations resulting from external interventions were detected Received in revised form 18 June 2015 by the imaging instruments such as the ﬂuorescence microscopy, the scanning/transmission electron Accepted 26 June 2015 microscopy (SEM/TEM) and the laser confocal microscopy. In this article, a new platform whi