adipose stem cells differentiated chondrocytes regenerate damaged cartilage in rat model of osteoarthritis.脂肪干细胞分化软骨细胞再生受损软骨骨关节炎大鼠模型.pdfVIP

Cell Biology International ISSN 1065-6995 doi: 10.1002/cbin.10596 RESEARCH ARTICLE Adipose stem cells differentiated chondrocytes regenerate damaged cartilage in rat model of osteoarthritis 1 1 1 2 1 Noreen Latief , Fahad Ali Raza , Fazal-Ur-Rehman Bhatti , Moazzam Nazir Tarar , Shaheen N. Khan and Sheikh Riazuddin1,2,3* 1 National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan 2 Allama Iqbal Medical College, University of Health Sciences, Lahore, Pakistan 3 Shaheed Zulfiqar Ali Bhutto University of Medical Sciences, PIMS, Islamabad, Pakistan Abstract Transplantation of mesenchymal stem cells (MSCs) or autologous chondrocytes has been shown to repair damages to articular cartilage due to osteoarthritis (OA). However, survival of transplanted cells is considerably reduced in the osteoarthritic environment and it affects successful outcome of the transplantation of the cells. Differentiated chrondroytes derived from adipose stem cells have been proposed as an alternative source and our study investigated this possibility in rats. We investigated the regenerative potential of ADSCs and DCs in osteoarthritic environment in the repair of cartilage in rats. We found that ADSCs maintained fibroblast morphology in vitro and also expressed CD90 and CD29. Furthermore, ADSCs differentiated into chondrocytes, accompanied by increased level of proteoglycans and expression of chondrocytes specific genes, such as, Acan, and Col2a1. Histological examination of transplanted knee joints showed regeneration of cartilage tissue compared to control