多态性观察初报-上海海洋大学学报.PDFVIP

第 17 卷第 1 期 上海水产大学学报 Vo l. l7 , No.l 2008 年 1 月 JOURNAL OF SHANGHAI FISHERIES UNIVERSITY Jan. ,2008 文章编号: 1004 -7271(2008)01 -0001 -05 团头勤倍间杂交三倍体性腺发育的 多态性观察初报 田刚，李思发 (上海水产大学农业部水产种质资源与养殖生态重点开放实验室，上海 200090) 摘 要:于5 月份团头妨正常繁殖期前夕，以初次性成熟(二足龄)二倍体团头筋为对照样本，同龄同池培育 的同源倍间正交兰倍体、同源倍间反交三倍体、异源倍间杂交三倍体为实验样本，分别取性腺，石蜡切片，镜检 发育状况。发现团头纺三倍体的性腺可归纳为三种情况: (1)性腺发育程度很低，表现为雄性二足龄三倍体 的性腺外观细小，生殖细胞处于精原细胞水平。 (2) 性腺发育出现异常，表现在性腺外观畸形，有卵巢单侧不 发育，有精巢大部分为细线，局部增粗等情况，还有发现外观元异常，但切片观察为雌雄嵌合体。 (3) 性腺发 育无异常，表现为性腺外观没有异常，切片观察也能观察到各期典型的生殖细胞。 关键词:团头妨;三倍体;性腺发育 中图分类号:S 917 文献标识码:A Primary report of observation on the diversity of gonad development in the triploid of blunt snout bream (Megalobrama amblycephala) crossbred by tetraploid and diploid TIAN Gang , LI Si-fa (Key Laboratory 0/Aquatic 由netic Resources and Aquaculture Ecosystem Certifìcated by the Ministry 0/Agriculture , Shanghai Fisheries University , Shanghα200090 ， China) Abstract :Just before the normal reproduction period of the blunt snout bream (Megαlobrama amblycephalα) in May , the gonad samples were collected from the first matured two-year-old diploid as control group , and from the communal raised triploid (crossbred by tetraploid and diploid , including auto-positive-triploid , auto- negative-triploid , allo-triploid) as checking group ,/gonads were sliced and observed under microscope. The gonad development of the gonads in triploid could be categorized into three status: (1) very poor develop ,for example the two-year-old male triploid s sperm町 we陀