一种基于聚乙二醇单甲醚稳定的纳米金可视化检测丙烯酰胺(AA)的新方法推荐.docxVIP

Preparation of Methoxy Polyethylene Glycol Stabilized Gold Nanoparticles and Their Applications for Detection and Absorption of Acrylamide摘　　要本文以聚乙二醇单甲醚(PEG)为原料，制备了聚乙二醇单甲醚纳米金（PEG-纳米金），并将聚乙二醇巯基功能化后制备了巯基化聚乙二醇单甲醚纳米金（PEG-HS-纳米金），然后将两种纳米金分别用于有毒小分子丙烯酰胺的检测和吸附上。建立了一种基于聚乙二醇单甲醚稳定的纳米金可视化检测丙烯酰胺(AA)的新方法，并用透射电子显微镜（TEM）、紫外可见光光度计(UV)、激光粒度分析仪(DLS)对聚乙二醇纳米金进行测试分析。结果表明：该聚乙二醇纳米金核呈分散均匀的规则球体，且其平均粒径为19nm。颗粒整体（纳米金核和表面稳定剂）粒径集中分布在40nm。浓度为0.004mg/mL的丙烯酰胺能使聚乙二醇纳米金发生团聚，胶体由红色变成紫色，浓度在0.004mg/mL-0.032mg/mL之间时，团聚程度随浓度的增加而增加，同时颗粒整体粒径依次增大到170nm、250nm、600nm。相同条件下，N, N-二甲基丙烯酰胺(DMAA)不能使聚乙二醇纳米金发生团聚。因此，可用该方法简单、快速的实现丙烯酰胺选择性检测。将巯基化聚乙二醇纳米金用于丙烯酰胺吸附，研究了其吸附性能、吸附机理以及循环利用。氢核磁（1H-NMR）、高效液相色谱（HPLC）、紫外可见分光光谱（UV）、透射电子显微电镜（TEM）、激光粒度分析仪（DLS）等测试表明：该巯基化聚乙二醇纳米金在有机溶剂和水溶液中均能吸附丙烯酰胺，但不吸附N, N-二甲基丙烯酰胺，这种吸附来源于丙烯酰胺的氢原子与巯基化聚乙二醇的氧原子之间的氢键。二氯甲烷中每个巯基化聚乙二醇纳米金平均吸附25772个丙烯酰胺分子，水溶液中每个巯基化聚乙二醇纳米金平均吸附22420个丙烯酰胺分子，比二氯甲烷中少，这可能是水溶液中水分子使丙烯酰胺与聚乙二醇之间的氢键作用减弱。另外，该巯基化聚乙二醇纳米金前4次循环团聚较少，循环5次之后，团聚量超过50%，这可能是多次离心使纳米金产出团聚引起的。因此，该巯基化聚乙二醇纳米金可能成为新型可循环利用的有毒小分子吸附剂。关键词：聚乙二醇；纳米金；丙烯酰胺；检测；吸附AbstractIn this paper, methoxy polyethylene glycol(PEG) stabilized gold nanoparticles（PEG-AuNPs）and thiol functionalized PEG stabilized gold nanoparticles (PEG-HS-AuNPs) have been prepared. Then these two gold nanoparticles were used in the detection and adsorption of the toxic small molecules, e.g. acrylamide, respectively.Firstly, the novel visual method for the detection of acrylamide by PEG-AuNPs has been developed. The PEG-AuNPs were characterized by transmission electron microscopy (TEM), ultraviolet spectrophotometer(UV), Dynamic Light Scattering (DLS). The results indicated that the size of the spherical AuNP core was 19 nm and well dispersed. In addition, the size of PEG-AuNPs (including Au cores and surface stabilizers) was around 40 nm. Significantly, 0.004 mg/mL acrylamide could induce the aggregation of the PEG-AuNPs, which caused the color changing of the colloidal solution from red to purple. The aggregations of AuNPs enhanced with the increase of acrylamide conce