microRNA_21在弥漫大B细胞淋巴瘤中的表达及意义.pdfVIP

临床与实验病理学杂志 J Clin Exp Pathol 2010 Apr; 26( 2) 163 m icroRNA-21 B 张继新, 吕晓涛, 高 颖, 崔力方, 张程燕, 昌 红,李保玉 : m iR-21 B ( diffuse large B-cell lymphoma, DLBCL) , m iR-21 DL- BCL DLBCL Rea-l tmi eR -PCR 36 DLBCL 10 m iR-21, SP K i-67P EN DLBCL m iR-21 DLBCL , 36 DLBCL 14 P EN( 389% ), 21 K i-6750% ( 583% )DLBCLm iR-21 P EN , DLBCL AnnA rbor(K i-6750% ) ( IPI) m iR-21 DLBCL, DLBCLP EN m iR-21 DLBCL : ; B; m icroRNA-21; R -PCR : R 733. 4 : A : 1001- 7399( 2010) 02- 0163- 05 Significance ofmicroRNA-1 expression in diffuse largeB-cell lymphoma ZHANG J-ixin, L X iao-tao, GAO ying, CU I L-i fang, ZHANG Cheng-yan, CHANG Hong, LI Bao-yu (Dep artm ent of Pathology, Beij ing Shij itanH osp ital, the N inth ClinicalM ed ical College of P eking University, B eij ing 00038, China) Abstract: Purpose o investigate the expression ofm iR-21 in d iffuse large B cell lym phom a and norm al lymph tissues and its poten- tial relevance w ith clinicopathological characteristics. M ethods In our study, the expression levels ofm iR-21 in 36 prmi ary DLBCL and 10 norm al lym ph node tissue specmi ensw ere exam ined by aqM an real-tmi e polym erase chain reaction. he expression of K i-67 and P EN w as detected by mi munoh istochem ical stain ing. Results he expression ofm iR-21w as sign ificantly h igher in tum or tissues than that in normal tissues. And itw as negatively correlatedw ith P EN. Up-regulatedm iR-21 expressionw as associatedw ith higher prolif- eration index (K-i6750% ) and advanced DLBCL AnnA rbor stage. Conclusions hese findings suggest that P EN is possibly one of the targets ofm iR-21 in DLBCL. Overexpression ofm iR-21 is a key feature that is correlatedw ith cellproliferation in DLBCL. M iR- 21 m ay ind icate a m ore aggressive phenotype and serve as a m olecu lar prognostic m arker in DLBCL. K ey ords: lym phom a; large B-cell; m icroRNA-2