取代-3-醛基吲哚对玉米大斑病病原菌室内活性测定毕业论文.docVIP

河 南 科 技 大 学 毕 业 设 计（论 文） 取代-3-醛基吲哚对玉米大斑病病原菌 的室内活性测定 姓 名 刘 洋 院 （系） 林学院 专 业　 植物保护 指导教师 车志平 2014年 5 月 30 日 取代-3-醛基吲哚对玉米大斑病病原菌的室内 活性测定 摘 要 玉米大斑病[Exserohilum turcicum (Pass.) Leonard et Suggs]是我国近几年玉米上发生的重要病害，其危害在一些地区已经超过玉米其它病害，成为玉米主要病害，对玉米产量造成的损失相当严重。因此，该病害的防治显得尤为重要。 吲哚（Indole）作为一种重要的精细化工原料，在农药生产研发中有着广泛的应用。许多由其衍生得到的农药产品或农药中间体具有良好的生物活性，如抗微生物活性。然而，取代-3-醛基吲哚对玉米大斑病病原菌的室内活性测定未见报道。鉴于此，本实验通过测定5种取代-3-醛基吲哚及阳性对照恶霉灵（Hymexazol）对玉米大斑病病原菌菌丝生长抑制活性，得到6条相应的毒力回归曲线及对应的EC50。吲哚-3-甲醛、6-甲基-3-醛基吲哚、4-甲基-3-醛基吲哚、5-硝基-3-醛基吲哚、5-氰基-3-醛基吲哚和阳性对照恶霉灵的EC50值分别为0.1240 mg/mL，0.5920 mg/mL，0.1870 mg/mL，0.0370 mg/mL，0.3292 mg/mL和0.0350 mg/mL。结果表明，取代基不同的3-醛基吲哚对玉米大斑病病原菌菌丝生长抑制存在显著差异，其中5-硝基-3-醛基吲哚表现出较其它四种化合物显著的抑菌活性，EC50值接近已商品化的农药恶霉灵。 通过本实验的研究，初步筛选出防治玉米大斑病较好的取代-3-醛基吲哚，为研发防治该病的新型药剂提供科学的理论依据。 关键词：取代-3-醛基吲哚；玉米大斑病菌；EC50；生长速率法 REPLACE - 3 - ALDEHYDE INDOLE INDOOR OF CORN BIG SPOT PATHOGEN ACTIVITY DETERMINATION ABSTRACT Corn big spot [Exserohilum turcicum (Pass) Leonard et Suggs] is our country the important diseases on maize in recent years. The harm in some areas has been more than corn other diseases, become a main disease, corn on corn yield loss is quite serious. As a result, the disease prevention and control is particularly important. Indole (Indole) as an important fine chemical raw materials, has been widely used in pesticide production research and development. Many pesticide products or pesticide intermediates which are obtained by its derivative has good biological activity,such as antimicrobial activity. However, replace-3-aldehyde indole on corn big spot pathogen reported for the first indoor activity determination. In view of this, this experiment by measuring five replace-3-aldehyde indole and positive control of the evil mildew (Hymexazol) of corn big spot pathogen hypha growth inhibitory activity, get 6 corresponding toxicity regression curve and the corresponding EC50.Methyl-3- aldehyde indole, 4-methyl-3-alde