关键词叶绿素蛋白质复合体低温胁迫瓠燃-广西植物.PDFVIP

维普资讯 4 —t7{ 广 西 植 物 Guihaia13(2)：174—179．May1993 光和暗条件下低温对黄瓜和水稻叶绿素 蛋 白质复合体的影响 主 、／／年晓萍 郭俊彦 S31『 (中国科学院华南植物研究所 ·广州 510650) 摘要 本试验以黄瓜和水稻幼苗为材料．研究了光照和黑暗条件下低温对植物叶绿素蛋自质 复台体的影响。SDS电泳结果表明：5℃及12h 28011otolm—ss’1处理2d．Chl一蛋 白质复合体的降解明显大于5℃暗低温处理；低温与光照对 P70o—CPa1的影响大于LHCP。 叶 绿素荧光测定表明i5℃及12h2801xmolm-2S叫 的处理对PSI的影响亦大于暗低温处理． 由 此认为：低温与光对植物叶绿体的PSI和PSI都有明显的影响．其机理可能与常温下高光强引 起的光抑制相类似；不同的是低温下中等光强就能引起光抑制。因此．在光照低温下往往加剧植 物冷害的发生 关键词叶绿素蛋白质复合体：低温胁迫 瓠 燃 THE EFFECT OF CHILLING STRESS0N CHL0R0PHYLL— PR0TElN COMPLEXES OF RICE AND CUCUMBER SEEDLINGS IN THE LIGHT AND DARK Liu Kengxi~ ，W ang Yirou,LiXiaop~agand GuoJunyan (South China InstituteofBotany，AcademiaSinica，Guangzhou 510650) Abstract In thiswork we used cucumber and rice seedlingsasexperimental ：materialsto study the changes ofchlorophyll-protein complexes in leaves after chilling trae tm entunderdark and light． Result indicated that the chlorophyll— proteinComahxeSweredecraesed in theesedlings exposed to 5℃ for2days． The Ieayestraeted with 5C and 280 molm—gs。1waadecreaesdmorethan thatelthe leavesunderdark at sametemperature．Effectofchilling and light interaction on P700--CPalwasgreat，Whlie itwaslesson LHCP． PSI activity was menitored by chlorophyllfluorescenceemission． Ttdeclined rap|dly at2Bo m01m’gS PFD， _五Ⅱtitdecreased slowlyatdark under Sam etemperature． Therefore，weconcludethatthis：injurymechanism wassimilartophotoinhibi- - toln by strong lightintensity． whlle photoinliibition m．duced by chilling tempera一 ~ture oc uld ac rry out e