连续校准对酶学指标质控的影响.docVIP

精品论文 参考文献 连续校准对酶学指标质控的影响 王书香 袁瑾 赛树玲 　　（遵义医学院附属医院医学检验科 贵州 遵义 563003） 　　【摘要】 目的：探讨连续校准和间断校准两种方法对酶学指标质控的影响。方法：连续校准5天，记录六项酶学指标ALT、AST、ALP、CHE、GGT、CK的吸光度OD值和质控值，观察质控稳定期长短，实验室信息管理系统（LIS)中记录质控结果。选取连续校准的40天酶学指标的质控结果，与间断性校准进行比较,观察校准后对酶学指标质控的影响。结果：AST间断校准后质控结果优于连续校准，ALP连续校准后质控结果好，ALT、GGT、CHE、CK连续校准和间断校准的质控结果没有显著性差异。结论：连续校准和间断校准两种方法对部分酶学指标质控没有影响。 　　【关键词】临床检验；校准；质控 　　【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2015）19-0041-02 　　 　　The influence of continuous calibration to quality control results for serum enzymes 　　Wang Shuxiang,Yuan Jin,Sai Shulin 　　Department of Clinical Laboratory,Affiliated Hospital of Zunyi Medical College,Guizhou Province,Zhunyi 563003,China 　　【Abstract】 Objective To investigate the effects on quality control (QC) for serum enzymes by continuous calibration and discontinuous calibration.Methods By continuous calibration for five days,the values of absorbance and quality control for six serum enzymes,such as ALT、AST、ALP、CHE、GGT 、CK were recorded.The stable period of QC was observed.QC results were recorded in Laboratory Information Management System(LIS).Compared with QC results of 40 days by discontinuous calibration and continuous calibration,the effect on QC results of serum enzymes was observed.Results For AST,QC results by continuous calibration is better than discontinuous calibration.ALP by discontinuous calibration is better than continuous calibration.There was no significant difference for ALT、GGT、CHE、CK.Conclusion There is no difference for partial serum enzymes by discontinuous calibration and continuous calibration. 　　【Key words】Clinical examination;Calibrate;Quality control 　　性的重要因素，即校准保证了患者样本的检测质量与参考标准的可比性。实验室校准的重点主要在于测量系统的校准，测量系统涉及到仪器、试剂、校准品及操作程序的组合，广义上还包括消耗品、检测用水、其他辅助设备、质量控制程序、维护保养程序、标本前处理程序以及培训合格的检验工作者。随着自动生化分析仪的普及应用，良好的仪器状态保证了高质量检验，稳定的试剂、严格按照标准操作规程执行检验的工作人员等因素确定后，校准品的溯源性、稳定性及校准次数成为影响结果准确性的重要因素[1]。实验室在检测患者标本前，每天将质控等同标本条件下进行检测，对校准有效性进行验证。 　　血清酶学项目的测定，在临床实验室已经广泛开展，是临床疾病诊断和疗效观察的重要指标。本科室采用封闭的测量系统，即试剂、