运动与左旋肉碱影响肥胖小鼠胆固醇代谢.docVIP

精品论文 参考文献 运动与左旋肉碱影响肥胖小鼠胆固醇代谢 南京体育学院运动健康科学系 江苏南京 210014 摘要：目的：探讨运动及左旋肉碱对胆固醇代谢的影响。方法：46只C57BL/6小鼠喂食高脂饲料，建立营养性肥胖模型。建模成功后将小鼠随机分为对照组（C组）、运动组（E组）、左旋肉碱组（L组）和左旋肉碱加运动组（LE组）4组。6周后采集血液、肝脏组织，检测血清和肝脏中的胆固醇水平及胆固醇代谢关键基因脂蛋白脂酶（LPL）、载脂蛋白E（ApoE）、低密度脂蛋白受体（LDLR）、CD36的基因表达。结果：运动与左旋肉碱补充均使肥胖小鼠体重显著降低（P lt; 0.05），而运动跟肉碱同时处理效果更佳。运动显著增高了血清中胆固醇和低密度脂蛋白的含量（P lt; 0.05）。运动结合肉碱补充显著提高了高密度脂蛋白的含量（P lt; 0.05）。运动与肉碱同时处理均显著上调LPL、ApoE和CD36的基??表达（P lt; 0.05）。单纯运动补充能显著提高LPL和ApoE的表达，同时显著降低LDLR的表达。结论：运动结合左旋肉碱补充通过刺激LPL、ApoE和CD36的表达调节胆固醇的水平，运动主要通过LPL、ApoE和LDLR调节胆固醇代谢，而单纯左旋肉碱补充对胆固醇代谢没有显著影响。 关键词：运动；左旋肉碱；胆固醇代谢 Abstract：Objective：To investigate the effects of exercise and L-carnitine on cholesterol metabolism.Methods：To establish nutritional obesity model by fedding high-fat diets to 46 C57BL/6 mice.Obesity mice were randomly divided into 4 groups，the control group（C），the exercise group（E），fedding L-carnitine group（L）and exercise combined L-carnitine supplementation group（LE）． 6 weeks later，mice were killed，and blood，liver tissue were collected．Lipids in serum and cholesterol metabolism key enzymes Low density lipoprotein（LDL），Apolipoprotein E（ApoE），Low density lipoprotein receptor（LDLR）and CD36 in liver were tested．Results：The body weights were significantly low in 3 experiment groups（P lt; 0.05），especially in exercise combined L-carnitine supplementation group．Total cholesterol and Low density lipoprotein in serum were significantly increased in exercise group（P lt; 0.05）． High density lipoprotein（HDL）in serum was significantly increased in the exercise combined L-carnitine supplementation group（P lt; 0.05）.The expression of LDL，ApoE and CD36 were significantly up in the exercise combined L-carnitine supplementation group（P lt; 0.05）.Pure sports supplement can improve the expression of LPL and ApoE，at the same time significantly reduced LDLR expression.Conclusion：Exercise combined with L-carnitine supplementary may regulate cholesterol level by stimulation expression of LPL，ApoE and CD36．Movement mainly reg