杆状病毒表达系统及其应用进展精选.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 10 韦永龙 李轶女 张志芳 沈桂芳 (, 100081) : 杆状病毒是节肢动物门的专性寄生病毒, 20世纪 80年代被 发为表达载体以来, 由于其真核表达环境等优 点, 受到了广泛的重视和研究, 短短不到 30年的时间里,杆状病毒表达体系的重组载体构建技术, 筛选技术得到了很大程度的 改进和简化, 成为与大肠杆菌酵母哺乳动物相并列的四大表达体系之一在表面展示, 类病毒颗粒表达, 哺乳动物基因转 移, RNA 干扰等方面取得了重要的成果就杆状病毒表达系统的发展及其应用作一 个综述 : 杆状病毒 BEVS 表达 Advances in Research and Application of Baculoviru s Expression System W eiYonglong LiY inv Zhang Zhifang Shen G ifang (B iotechnology Research Institute, CAAS, B eijing 100081) Abstrac:t Bac lovir ses are ob ligate lethal pathogens of arthropodas. They have been h igh ly val ed and w idely st d ied since be ing exploited as a expression vector in 1980s, for their excellen t advantage incl d ing e karyotic express ion enviroment. In less than 30 years, great advances have ach ieved involving recom b inant bac lovir s constr ction and screening. Th s, the bac lovir s expression vec tor system ( BEVS) has becom e one of the fo rm ost pop lar expression system com prising bacteri m, yeasts, m ammalian cells and bac lovir ses. The BEVS has been sed in s rface d isplay, vir slike particles prod ction, m amm alian cells gene delivery, RNA interfer ence, etc. The developm ent and application of the BEVS is reviewed in this article. K ey w ords: Bac lovir s BEVS Express ion DNA , ( 80- 160 kb ) , 600 , [ 1] , , 7 , , , ( n cleopolyhedrovir s, NPV ) , ( gran lovir s, GV ) [ 2] , , , ( bac lovir s expression vector system, BEVS) , , , , 1 , , , Sm ith GE , 1983A cNPV , , 30, , , : : ( 30770