基因的分子克隆、鉴定及免疫相关性表达.PDF

46 6 Vol.46, No.6 2 0 1 5 1 1 OCEANOLOGIA ET LIMNOLOGIA SINICA Nov., 2015 (Plecoglossus altivelis)-α (TNF-α) * ( 315211) -α(tumor necrosis factor-alpha, TNF-α)TNF , (Plecoglossus altivelis)/ , TNF-α(PaTNF-α)cDNA PaTNF-α 1932 , , 235 , 26.4 kDa , PaTNF-α TNF 1 TACE 1 , (Oncorhynchus mykiss)TNF-α, 53.4%; , TNF-α , PaTNF-αTNF-αPCR (quantitative real-time PCR, qRT-PCR) , PaTNF-α mRNA ; ( Vibrio anguillarum) PaTNF-α mRNA ; /LPS poly(I: C) , PaTNF-α mRNA PaTNF-α, Western blot , /PaTNF-α , TNF-α mRNA , TNF-α TNF-α; ; ; /; Q786; S917 doi: 10.11693/hyhz20150600166 -α(tumor necrosis factor-alpha, (Van Horssen et TNF-α), al, 2006; Horiuchi et al, 2010; Willrich et al, 2015) , TNF-α (Horiuchi et al, 2010), TNF-α , (Oncorhynchus mykiss) (Laing et , , al, 2001; Hong et al, 2013)(Cyprinus carpio) , (Savan et al, 2004)(Psetta maxima) (Ordás et (Horiuchi et al, 2010; Rajmani et al, 2015); al, 2007)(Siniperca chuatsi) (Xiao et al, 2007) , TNF-α (Ctenopharyngodon idella) (Zhang et al, 2012) , -1 (interleukin-1, IL-1) TNF-α, -6 (interleukin-1, IL-6) , ( Vibrio pelagius) (Gruys et al, 2005; Horiuchi et al, 2010) (Viral haemorrhagic