微生物降解羟基苯甲酸的研究进展-微生物学报.PDF

微生物学通报 Oct. 20, 2014, 41(10): 2134−2142 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140033 专论与综述 微生物降解4-羟基苯甲酸的研究进展 李晶晶 朱莉 张晓琰 田俊 冯昭中 彭学* ( 221116) 摘 要4-羟基苯甲酸(4HBA)是在自然界中广泛存在的芳香族化合物，也是很多天然产物和人工合 成化合物的中间代谢产物。4HBA 的代谢途径有原儿茶酸开环途径、脱碳酸途径和厌氧微生物的苯 甲酰-CoA 还原途径，以及尚未完全阐明的龙胆酸开环途径。从4HBA 转化为龙胆酸的过程包含NIH 重排反应步骤，本综述重点介绍NIH 重排反应的研究进展并初步介绍了涉及 4HBA 降解过程中的 酶。在本综述中，结合我们的研究工作介绍了一个嗜热Bacillus sp. B1 菌株降解4HBA 等芳香族化 合物的代谢途径，最后对4HBA 降解过程中的NIH 重排反应研究进行了展望。 关键词4-羟基苯甲酸，NIH 重排反应，龙胆酸，嗜热芽孢杆菌 Recent advances in microbial degradation of 4-hydroxybenzoate a review * LI Jing-Jing ZHU Li ZHANG Xiao-Yan TIAN Jun FENG Zhao-Zhong PENG Xue (College of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China) Abstract: 4-Hydroxybenzoate (4HBA) is a naturally occurring aromatic compound, and as a key intermediate metabolite not only for natural products but also for artificial products. There are four 4HBA metabolic pathways: protocacechuate cleavage pathway; catechol cleavage pathway; anaerobic degradation pathway in anaerobes; gentisate cleavage pathway. The last pathway including a NIH shift reaction remains to be elucidated. In this review we emphasized in NIH shift reaction involved in the 4HBA degradation. The key enzymes of each 4HBA metabolic pathway also be introduced. Finally, we described thermophilic Bacillus sp. B1 strain which was capable of degradation of various aromatic compounds including 4HBA, and