有哪些信誉好的足球投注网站An Integrated Amperometric Biosensor for the Determination of Lactose in Milk and Dairy Products英文电子书.pdfVIP
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J. Agric. Food Chem. 2010, 58, 7141–7148 7141 DOI:10.1021/jf101173e An Integrated Amperometric Biosensor for the Determination of Lactose in Milk and Dairy Products F. CONZUELO, M. GAMELLA , S. CAMPUZANO , M.A. RUIZ, A. J. REVIEJO, AND J. M. PINGARRON *´ Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040, Madrid, Spain An integrated amperometric biosensor for the determination of lactose is reported. The bioelectrode design is based on the use of a 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM)- modified gold electrode on which the enzymes β-galactosidase (β-Gal), glucose oxidase (GOD), peroxidase (HRP) and the mediator tetrathiafulvalene (TTF) are coimmobilized by a dialysis mem- brane. β-Gal catalyzes the hydrolysis of lactose, and the produced glucose is catalytically oxidized to gluconic acid and H O , which is reduced in the presence of HRP. This enzyme reaction is 2 2 mediated by TTF, and the reduction of TTFþ at 0.00 V (vs Ag/AgCl) gives rise to an amperometric signal proportional to the lactose concentration. The biosensor exhibits a good repeatability of the measurement carried out with the same biosensor, a good reproducibility of the responses obtained
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