ION EXCHANGE CHROMATOGRAPHY AND ELECTROPHORESIS OF EGG YOLK PROTEINS英文电子书.pdfVIP

ION-EXCHANGE CHROMATOGRAPHY AtiD ELECTROPHORESIS OF EGG YOLK PliOTiINS L. E. MC BEE and 0. J. COTTERILL ABSTRACT METHODSMATERIALS Pure egg yolk was fractionated using DEAE ion-exchange cellulose. Chromatographic procedure Eluted components were simultaneously. detected by fluorescence and ultra-violet absorption. Selected chromatographic fractions were ,A continuous gradient system was formed by permitting buffer subjected to disc gel electrophoresis and assayed for total lipids, from an upper reservoir to flow into a closed mixing chamber at the amino acids and phosphorus. Native egg yolk was separated into 18 same rate it was removed. This elution buffer passed consecutively peaks chromatographically and 23 bands electrophoretically. Multi- (3 ml per min) through a pH monitor, a UV reference flow cell, component chromatographic peaks were demonstrated by electro- metering pump, ion-exchange column, UV monitoring cell, fluoro- phoresis. Chromatographic peaks and corresponding electrophoretic metric monitoring cell, volumeter and then to a fraction collector. bands.were identified by literature reference for lipovitellin, livetins, Aliquots of 20 ml were collected. An ISCO Model UA-2, double phosvitin and a low density fraction. A direct relationship existed beam, ultraviolet absorba