烟草黑胫病拮抗放线菌的分离与筛选.PDFVIP

中国烟草科学 2007 ，28 （5 ）：5-8 5 烟草黑胫病拮抗放线菌的分离与筛选 1 2 1 1 1 3 1 张永春 ，黄 镇 ，杜怀敏 ，陆 宁 ，谢已书 ，王 彬 ，关国经 （1.贵州省烟草科学研究所，贵阳 550003 ；2. 中国科学技术大学烟草与健康研究中心，合肥 230052 ；3. 山东中烟工业公司 济南卷烟厂，济南 250100 ） 摘 要：从堆沤过圈肥的土壤中采集样品，用平板法对所采样品进行菌株分离、纯化培养，共获得原始放线菌 101 株，再通 过室内拮抗试验，从随机 28 株试验菌株中初步获得 16 株具有一定拮抗作用的菌株。对拮抗效果较好的 6 株菌株以及抑菌圈较 小、但其菌落较大的 A15 菌株进一步做盆栽防治试验。结果表明，A6 的防治效果最好，达到55.26%，其它菌株效果一般。 关键词：烟草黑胫病；放线菌；拮抗 中图分类号：S572.062 文献标识码：A 文章编号：1007-5119(2007)05-0005-04 Isolation and Screening of Antagonistic Actinomyces against Phytophthora parasitica var. nicotianae Tucker 1 2 1 1 1 3 1 ZHANG Yongchun , HUANG Zhen , DU Huaimin , LU Ning , XIE Yishu , WANG Bin , GUAN Guojing (1.Guizhou Tobacco Research Institute, Guiyang 550003, China; 2. University of Science and Technology of China, Hefei 230052, China; 3. Jinan Cigarette Factory, China Tobacco Shandong Industrial Corporation, Jinan 250100, China) Abstracts: 101 strains of original Actinomyces spp. were isolated from soils composted with barnyard manure, and 16 strains with definite antagonistic effect were selected from 28 random strains by laboratory antagonistic test. The results of pot-culture control test using 6 strains with good antagonistic effect and A15 strains with less inhibition zone and greater colony showed that prevention effect of A6 strain was the best, up to 55.26%, and that of the other strains was less. Keywords:phytophthora parasitica var. nicotianae; Actinomyces spp; antagonism 烟 草 黑 胫 病 [Phytophthora parasitica var. 获得黑胫病生物防治的新途径。 nicotianae （Breda de Hann ）Tucker]是烟草生产上重 1 材料与方法